中文摘要：

目的：应用液相色谱-质谱联用法对葡萄籽提取物中的组成成分进行测定。方法：采用Zobax SB-C18色谱柱（250 mm×4.6 mm,5μm）,流动相为乙腈（A）-水（1%甲醇＋0.1%甲酸）（B）,梯度洗脱,流速为1.0 m L·min^-1,柱温为40℃,进样体积10μL;电喷雾电离（ESI）,毛细管温度300℃,碎裂能量30%,离子源电压4.5 k V,毛细管电压12.7 V,对葡萄籽提取物中的组分定性分析。结果：应用LC-MS法明确了葡萄籽提取物的组分主要为单体和原花青素低聚体,并分析二级质谱碎片推测出了原花青素的裂解规律。结论：应用液相色谱-质谱联用技术可以较好地对葡萄籽提取物中没食子酸、儿茶素、表儿茶素等组分进行测定。

英文摘要：

Objective： To apply an LC-MS method for determination of components in grape seed extract. Methods： Chromatographic separation was performed on an Agilent zobax SB -C18 column （ 4.6 mm×250 mm, 5 μ m ）, the mobile phase was acetonitrile-water （ 1% methanol＋0.1% methanoic acid ）with the gradient elution at a flow rate of 1.0 mL. min^-1 ; the column temperature was maintained at 40 ℃, and the injection volume was 10 μ L. The qualitative analysis of grape seed extract components was carried out by electrospray ionization （ ESI ）under the following condition： capillary temperature was at 3ou u, me ractuly was 4.5 kV, and capillary voltage was 12.＇7 Y. Results：The application of LC-MS proved the composition of grape seed extract mainly as monomers, oligomers and procyanidins and secondary mass spectrometry analysis of debris infer the pyrolysis regularity of procyanidins. Conclusion： Gallic acid, catechin and epicatechin components in grape seed extract can be better determined by the application of LC-MS.