中文摘要：

目的：建立RP-HPLC法同时测定葡萄籽提取物中没食子酸、儿茶素、表儿茶素3种单体的含量。方法：采用Agilent zobax SB-C18色谱柱（4.6 mm×250 mm,5μm）,以乙腈（A）-水（1%甲醇＋0.15%三氟乙酸）（B）为流动相梯度洗脱（0~10min,0%A→7%A;10~30 min,7%A→16%A;30~40 min,16%A→90%A;40~50 min,90%A;50~55 min,90%A→0%A;55~65 min,0%A）,流速1.0 m L·min^-1,检测波长280 nm,柱温40℃,进样体积20μL。结果：没食子酸、儿茶素和表儿茶素3种单体质量浓度分别在1.0~54.0μg·m L-1（r=0.999 8）、2.7~136.0μg·m L-1（r=0.999 8）、2.7~136.0μg·m L-1（r=0.999 9）范围内与峰面积呈良好的线性关系;平均回收率（n=6）分别为100.3%、99.15%和100.3%。3批GSE样品中没食子酸的含量分别为0.69%、0.69%、0.68%,儿茶素的含量分别为3.15%、3.12%、3.16%,表儿茶素的含量分别为2.48%、2.44%、2.42%。结论：建立的HPLC法经方法学验证,可用于葡萄籽提取物中没食子酸、儿茶素、表儿茶素含量的同时测定。

英文摘要：

Objective： To establish an RP- HPLC method for simultaneous determination of three monomers（ gallic acid,catechin,epicatechin） in extracts of grape seeds. Methods： Chromatographic separation was performed on the Agilent zobax SB- C18column（ 4. 6 mm × 250 mm,5 μm）. The mobile phase was acetonitrile（ A） – water（ 1% methanol ＋ 0. 15% TFA）（ B） with the gradient elution（ 0^-10 min,0% A →7% A; 10- 30 min,7% A →16% A;30- 40 min,16% A→90% A; 40- 50 min,90% A; 50- 55 min,90% A→0% A; 55- 65 min,0% A）,at the flow rate of 1. 0 m L·min^-1. The detective wavelength was set at 280 nm,the column temperature was maintained at 40℃ and the injection volume was 20 μL. Results： The linear ranges were 1. 0- 54. 0 μg·m L^-1（ r =0. 999 8） for gallic acid,2. 7^-136. 0 μg·m L^-1（ r =0. 999 8） for catechin and 2. 7-136. 0 μg·m L^-1（ r =0. 999 9） for epicatechin. The average recoveries（ n = 6） of the three monomers were 100. 3%,99. 15% and 100. 3%. The contents of gallic acid,catechin and epicatechin in three lots of GSE samples were respectively 0. 69%,0. 69%,0. 68%; 3. 15%,3. 12%,3. 16%; and 2. 48%,2. 44%,2. 42%. Conclusion： Through method validation,the developed HPLC method is suitable for the simultaneous determination of gallic acid,catechin and epicatechin in extracts of grape seeds.