中文摘要：

【目的】中国地方猪种在抗病性和免疫力方面优于国外猪种,但是对中外猪种抗病力差异的遗传基础和免疫机理却了解甚少。有推测不同品种猪对疾病的抵抗力不同可能在细胞因子和受体的表达水平上有所反映。本研究以抗病力强的大蒲莲和抗病力相对较弱的长白猪的断奶仔猪为研究对象,对6个具有重要功能的细胞因子（促炎症细胞因子（IL8和TNFα）,抗炎症细胞因子（IL10）,干扰素调节因子（IRF3）和干扰素（IFNA和IFNG））和1个最具代表性的模式识别受体（TLR4）基因在大蒲莲和长白仔猪群体外周血液中的基因表达量进行检测,并对各基因进行品种和母猪效应分析,为了解中外不同猪种的免疫水平奠定基础。【方法】采集大蒲莲（104头）和长白（171头）35日龄仔猪的外周血,提取总RNA,逆转录为cDNA后,利用荧光定量PCR法,首先对6个常用的候选内参基因（ACTB、GAPDH、B2M、TBP、RPL4和PPIA）利用geNorm软件进行稳定性评价分析,筛选仔猪外周血RNA基因定量分析中稳定表达的内参基因,然后利用该内参基因进行校正,对上述细胞因子和模式识别受体基因在大蒲莲和长白仔猪群体外周血中的表达量进行定量,并利用最小二乘法对细胞因子和受体在大蒲莲和长白仔猪群体中的表达量进行品种和母猪效应分析。【结果】研究结果归纳为4个方面：1.外周血样品中内参基因稳定性值M从大到小依次为：ACTB（0.595）〉GAPDH（0.571）〉PPIA（0.469）〉RPL4（0.466）〉TBP（0.461）〉B2M（0.440）,选择表达稳定性最高的基因B2M作为内参基因对细胞因子和受体基因的表达量进行校正。2.检测的6个细胞因子的基因表达量存在很大的差异,各细胞因子基因表达量ΔCt最大值和最小值之间差值分别为9.23—14.25和5.83—8.73;受体TLR4的ΔCt值的最大和最小值之间相差相对较小,大蒲莲和长白仔猪分别为5.03?

英文摘要：

Objective] Though Chinese indigenous breeds have stronger disease resistance and immunity than imported commercial breeds, little is known about the genetic basis and immune mechanism of their difference. It is supposed that the gene expression level of cytokines and receptors may be the reasons leading to the different disease resistances among breeds. In the present study, the mRNA expression levels of seven genes in piglets of Dapulian and Landrace were determined, including two pro-inflammatory cytokines （IL8 and TNFα）, one anti-inflammatory cytokine （IL10）, one interferon regulatory factor （IRF3）, two interferon （IFNA and IFNG） and one pattern recognition receptor （TLR4）, and their breed and sow effects in the two breeds were analyzed, which would benefit the understanding of the immunity levels of Chinese indigenous breeds and the commercial breeds.[Method] Blood samples of clinically healthy Dapulian （n=104） and Landrace （n=171） piglets of 35 days old were collected, and their total RNA was extracted and reverse-transcribed into cDNA. Based on the quantitative real-time PCR, firstly, the expression stabilities of the six candidate genes （ACTB, GAPDH, B2M, TBP, RPL4 and PPIA） were evaluated by the geNorm program to select the most stable reference gene. Then, the selected reference gene was used for normalization, and the gene expression levels of the above mentioned cytokine and receptor genes were quantified in piglets of Dapulain and Landrace. Finally, the breed and sow effects of their expression levels in the two breeds were analyzed by Least Square Means. [Result] The expression stability values （M） of the six candidate reference genes in blood were as follows： ACTB （0.595） ＆gt; GAPDH （0.571） ＆gt; PPIA （0.469） ＆gt; RPL4 （0.466）＆gt; TBP （0.461） ＆gt; B2M （0.440）. B2M, which had the most stable expression, was selected as the reference gene. In Dapulian and Landrace, the D-values between the maximum and minimum of ΔCt of the six cy