中文摘要：

目的研究地塞米松（DEX）诱导的小鼠胸腺细胞凋亡中线粒体质量变化。方法分别设对照组（Control）和DEX组；在6h，分别以NAO和Mitotracker Green（MG）单染流式细胞术检测线粒体质量变化，以DiOC6（3）染色流式细胞术检测线粒体膜电势变化，以Annexin V-PE／MG双染流式细胞术检测细胞凋亡中线粒体质量改变特点。结果NAO染色检测结果显示终浓度1μmol·L^-1 DEX可以诱导小鼠胸腺细胞线粒体心磷脂含量降低，与对照组比较P〈0．01；MG染色流式细胞术结果表明DEX（P〈0．01）诱导了胸腺细胞线粒体质量的降低；DEX（P〈0．01）诱导线粒体膜电势依赖性DiOC6（3）在胸腺细胞中可染性下降；Annexin V-PE／MG双染流式细胞术显示，凋亡细胞中存在一定比例的低线粒体质量细胞，DEX组该群细胞多于对照组（P〈0．01）。结论DEX诱导小鼠胸腺细胞凋亡中线粒体质量降低；线粒体质量评价在细胞凋亡相关药理学研究领域有应用前景。

英文摘要：

Aim To study on the mitochondrial mass change in mouse thymocyte apoptosis. Method Control and Dexamethasone （DEX） groups were set; at 6h, and and we studied mitochondrial mass changes by NAO Mitotracker Green （ MG ） staining flowcytometry detected mitochondrial membrane potential change with DiOC6 （3） staining flowcytometry. We also used Annexin V-PE/MG double staining flocytometry to examine the mitochondrial changes in apoptosis progress. Results NAO staining results showed that 1 μmol · L^-1 DEX stimulation reduced the cardiolipin content of thymocyte mitochondria （P 〈 0.01 ）. MG staining confirmed the mitochondrial mass decline with DEX treatment （P 〈0. 01 ） ,and also found DEX reduced the DiOC6 （3） staining efficiency in thymocytes （P 〈 0. 01 ）. Annexin V-PE/MG staining results showed that cell population with low mitochondrial mass existed in apoptotic thymocytes, and they were more in DEX group than in control group （P 〈 0.01 ）. Conclusion Mitochondrial mass declined in DEX induced mouse thymocyte apoptosis; mitochondrial mass evaluation is promising for the application to the apoptosis related immunological pharmacy study.