中文摘要：

直接用可溶性Jagged-1／Fc嵌合蛋白（Jagged-1／Fc）在体外诱导小鼠淋巴结细胞向CD4^＋CD25^＋T细胞分化．通过荧光标记单克隆抗体染色结合流式细胞术，观察不同剂量Jagged-1／Fc在不同时间对淋巴结细胞向CD4^＋CD25^＋T细胞分化的影响，观察Jagged-1／Fc诱导T细胞内细胞因子的变化；藉ELISA法检测Jagged-1／Fc诱导分化的T细胞分泌TGF-β1、IL-4和IL-10的水平．结果显示，超过500．0μg/L剂量的Jagged-1／Fc使CD4^＋CD25^＋T细胞百分比明显增高，诱导时间需要4～6天，抗Jagged-1单抗能抵消Jagged-1／Fc的诱导作用，用DAPT阻断Notch信号通路的活化也能抑制Jagged-1／Fc的诱导作用，Jagged-1／Fc诱导分化的T细胞培养上清中IL-4和IL-10的水平明显增高，TGF-β1无明显变化，胞内IL-4，IL-10，IL-2和TNF-α的水平也呈增高趋势．上述结果表明，可溶性Jagged-1／Fc嵌合蛋白在体外可诱导小鼠淋巴结细胞向CD4^＋CD25^＋调节性T细胞分化．

英文摘要：

A soluble Jagged-1/Fc chimera protein （Jagged-1/Fc） was directly used to induce the differentiation of lymphonode cells in mice into CD4^＋CD25^＋ regulatory T cells in vitro. A fluorescein-labeled monoclonal antibody staining combined with flow cytometry was applied to detect the effect of different doses of Jagged- 1/Fc on the differentiation of the lymphonode cells into CD4^＋CD25^＋ T cells at different time, and to measure intracellular cytokine changes of the T cells induced by Jaggde1/Fc. The level of TGF-β1, IL-4 and IL-10 secreted by the T cells that were induced by Jagged-1/Fc was determined by ELISA. The results showed that over 500.0 μg/L of Jagged-1/Fc led to the obvious enhancement of the proportion of CD4^＋CD25^＋ T cells within the day 4 to 6 of induction, which was abrogated with an anti-Jagged-1/Fc monoclonal antibody. This induction action of Jagged-1/Fc on CD4^＋CD25^＋ T cells was also inhibited by the block of a Notch signal pathway with N- [N- （3,5-difluorophenacetyl）-L-alanyl]-S-phenylglycine t-butyl ester （DAPT）. The level of IL-4 and IL-10 in the supernatant of T cell culture and their intracellular level were elevated by the induction of Jagged-1/Fc, and the level of TGF-β1 in the supematant was not altered. These findings suggest that a soluble Jagged-1/Fc chimera protein may induce the differentiation of mouse lymphonode cells into CD4^＋CD25^＋ regulatory T cells in vitro.