中文摘要：

背景：天麻素广泛应用于眩晕、头痛及高血压等的辅助治疗，其作为干预物质用于高脂肥胖大鼠的治疗有待评估。 目的：构建不同浓度天麻素对高脂饮食性肥胖大鼠模型，观察其对体质量与血清代谢物水平的影响，分析其可能的作用机制。 设计：随机对照动物实验。 单位：兰州大学基础医学院生理与心理研究所。 材料：实验于2007—06／2007—08在兰州大学基础医学院生理与心理研究所和甘肃省新药临床前研究重点实验室完成。选用44只生后1周雄性健康SD大鼠，体质量（99．57±2．13）g，由中科院上海斯莱克实验动物有限公司提供。实验过程中对动物的处置符合动物伦理学标准。基础饲料由苏州双狮实验动物饲料科技有限公司生产，高脂饲料由本实验室自行配制。每100克含基础饲料57．5g、蛋黄粉11．79g、猪油10g、猪胆盐0．2g、酪蛋白7g、奶粉13g、食盐0．085g、酵母粉0．425g：其中含脂肪22．07g、蛋白质23．7g、碳水化合物39g、热量472．16千卡。纯度为98％的天麻素购自陕西旭煌植物科技开发公司。丙二醛和总抗氧化能力试剂盒均为南京建成生物工程研究所生产。 方法：构建天麻素干预高脂饮食性肥胖大鼠模型：①采用基础饲料适应喂养大鼠l周，随机数字表法将大鼠分为正常对照组（n=10）、高脂组（n=10）、高脂＋天麻素低、中、高剂量组（每剂量组各8只）。②正常对照组喂基础饲料，其它各组均喂高脂饲料。正常对照组和高脂组每天灌胃0．9％生理盐水0．3mL，高脂＋天麻素低、中、高组灌胃同等容量浓度分别为15，30，60mg／（kg·d）的天麻素溶液。③各组大鼠自由进食和饮水，共8周。 主要观察指标：①每周末检测各组大鼠体质量。②每日上午定时记录大鼠摄入热量。③高脂喂养结束后，于大鼠股动脉取血测血糖值，同时检测血?

英文摘要：

BACKGROUND： Gastrodin （GAS） is widely used as adjuvant therapy for vertigo, headache and hypertension. However, it is recently noticed that GAS might be used as an agent for treating obesity. OBJECTIVE： To set up obese rats of high-fat diet to observe the effects of different concentrations of GAS on body mass and serum metabolite levels and to analyze its possible mechanism. DESIGN： A randomized and controlled animal experiment. SETTING： Institute of Physiology and Psychology, School of Basic Medical Science, Lanzhou University. MATERIALS： This study was performed at the Institute of Physiology and Psychology, School of Basic Medical Science, Lanzhou University and Gansu Provincial Key Laboratory of Pre-clinical Study for New Drugs from June to August in 2007. Forty-four healthy one-week-old male SD rats, weighing （99.57±2.13） g, were purchased from Shanghai SILAIKE Laboratory Animal Co., Ltd. Disposal of animals was in accordance with the animal ethics standards. Basic animal feed was provided by Suzhou Shuangshi Laboratory Animal Feed Science and Technology Co., Ltd. High-fat forage were self-made in the authors＇ laboratory. Each 100 gram of high-fat forage consisted of basic feed （57.5 g）, egg yolk powder （11.79 g）, lard （10 g）, pig bile salt （0.2g）, casein （7g）, milk power （13 g）, salt （0.085 g）, and yeast powder （0.425 g）, and the 100 gram of high-fat forage contained of fat （22.07 g）, protein （23.7 g）, carbohydrate （39 g）, and quantity of heat （472.16 calorie）. GAS （98% in purity） was purchased from Shaanxi Xuhuang Botanical Science ＆ Technology Development Co., Ltd. Malondialdehyde （MDA） and total antioxidative capability （T-AOC） kits were purchased from Jiancheng Bioengineering Institute, Nanjng, Jiangsu Province. METHODS： After being adaptively fed with basic animal feed for one week, all SD rats were randomly divided into five groups： normal control group （NC, n = 10）; high-fat-fed control group （HFFC, n