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中文摘要：
利用DNA条形码技术对中药威灵仙及其伪品进行鉴别，为威灵仙分子鉴定提供更多依据与参考。本实验利用psbA．trnH序列对来自3个属10个物种的72份威灵仙及其伪品样本进行扩增测序，结合GenBank数据库中7个属21个物种的284条序列，分析ITS、ITS2、psbA．trnH、rbcL、matK各序列种内与种间变异、遗传距离、barcodinggap，并采用最近距离法（nearestdistance）和相似性搜索算法（BLAST1）评价5条序列的鉴定成功率。结果显示psbA．trnH序列在两种DNA序列鉴别评价方法中的效率均较高，且该序列种间变异较大，在barcodin2gap检验中，其序列重叠区较少，种间与种内变异分布呈两边分开的趋势，NJ树分析可将各物种分开，差异显著。综合鉴别效率和扩增成功率，推荐使用psbA—trnH序列作为威灵仙药材及其伪品的鉴别序列。
英文摘要：
This study provides the candidate sequences in the identification of Radix et Rhizoma Clematidis and its adulterants using DNA barcoding. We amplified and sequenced the region psbA-trnH, with the data of 284 sequences from GenBank, the differential intra- and inter-specific divergences, genetic distance, barcoding gap were used to evaluate five barcodes, and the identification efficiency was assessed using BLAST1 and Nearest Distance methods. The results showed that psbA-trnH barcodes performed high identification efficiency and inter-specific divergences among the five different DNA barcodes. Analysis of the barcoding gap and NJ tree showed psbA-trnH was superior to other barcodes. Based on the identification and PCR amplification efficiency, psbA-trnH can be the ideal barcode to identify Radix et Rhizoma Clematidis and its adulterants accurately.
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