中文摘要：

目的比较5个基源6个品种川贝母防治小鼠复发性哮喘的疗效。方法 81只雌性昆明小鼠按体质量均衡随机分为9组：模型组、对照组、地塞米松阳性对照组、6个品种川贝母醇提物给药组。除对照组外,其他各组小鼠于第1、7、14天sc卵白蛋白佐剂致敏,第22～28天以卵白蛋白生理盐水溶液雾化发敏。在造模同时,地塞米松组每天给药1次,每次0.6 mg/kg;川贝母醇提物每天给药1次,每次生药830 mg/kg均连续给药28 d,对照组给药同量0.1%羧甲基纤维素。检测肺泡灌洗液表面张力;HE染色法镜下观察肺组织病理变化,形态计量细支气管狭窄和肺泡体积差。结果与对照组比较,模型组小鼠气管狭窄,肺泡体积差与肺洗液表面张力增加（P〈0.01）;细支气管管壁充血、水肿、炎细胞浸润,腺体增生,黏液分泌亢进。川贝母各组小鼠病变减轻（P〈0.01）;在增加肺表面活性物质、维持肺表面张力与肺泡体积差异上,暗紫贝母（松贝）和梭砂贝母作用突出;在减轻细支气管狭窄上,暗紫贝母（松贝）和太白贝母作用突出;减少炎性细胞浸润上,暗紫贝母（松贝）和甘肃贝母作用突出;在减轻腺体增生、内膜增厚上,暗紫贝母（松贝）和太白贝母作用突出。总体效果以暗紫贝母（松贝）最好。结论 6种川贝母在防治小鼠复发哮喘总体效果和作用环节上各有特色,应保护药用川贝母的品种多样性。

英文摘要：

Objective To compare the curative effect of Fritillariae Cirrhosae Bulbus （FCB） in six species from five origins on recurrent asthma in mice. Methods Eighty one female Kunming mice were randomly divided into nine groups with equal weights, such as model, control, Dexamethasone （DEX, positive control）, and six FCB groups. The mice in the model, DEX, and FCB groups were sc injected with ovalbumin （OVA） on days 1, 7, and 14, then challenged with the aerosols of normal saline contained OVA on days 22--28. The mice in DEX and FCB groups were ig administered with DEX （0.6 mg/kg） and FCB （830 mg/kg） respectively for 28 d, and the mice in the control group were given 0.1% carboxymethyl cellulose. The surface tension ofbronchoalveolar lavage was measured, the lung tissues were taken for hematoxylin-eosin （HE） staining, and the tracheal stenosis and volume difference of pulmonary alveolus were analyzed by the microphotograph. Results Compared with the control group, the tracheal stenosis, surface tension, and volume difference of pulmonary alveolus were increased obviously in the model group （P 〈 0.01）. The pathological section revealed vessel wall thickening in bronchiole, inflammatory cell infiltrating, gland hyperplasia, and mucus hypersecretion, but FCB could reverse these pathological changes （P 〈 0.01）. Each FCB had its feature to cure asthma. F. unibracteata and F. delavayi had the most potency on increasing alveolar surfactant; F. unibracteata and E taipaiensis had the most potency on attenuating tracheal stenosis; F. unibracteata and E przewalskii had the most potency on relieving the inflammation; E unibracteata and F. taipaiensis had the most potency on reducing glandular hyperplasia or intimal thickening. Each FCB had its feature to cure asthma, but E unibracteata was the best. Conclusion FCB has the best potency to prevent and cure the recurrent asthma in mice, but each FCB has its feature.The diversity of FCB species should be protected.