中文摘要：

目的本实验采用超高效液相色谱法（UPLC）建立同时测定主产地（甘肃、陕西、内蒙古）黄芪药材中毛蕊异黄酮苷，芒柄花苷，槲皮素，毛蕊异黄酮，山柰酚，芒柄花素6种黄酮类成分的含量测定方法。方法采用ACQUITYUPLCBEHC18（2．1mm×100mm，1．7μm），流动相A为0．1％甲酸水，流动相B为0．1％甲酸乙腈一异丙醇（7：3），流速为0．25mL·min-1，梯度洗脱，检测波长254nm，柱温30℃，进样量2μL。结果6种黄酮类成分在选定的范围内线性关系良好（r≥0．999），平均加样回收率（n=6）在99．60％～101．2％，RSD1．2％～2．0％。结论本实验首次采用UPLC对黄芪中毛蕊异黄酮苷、芒柄花苷、槲皮素、毛蕊异黄酮、山柰酚、芒柄花素6种黄酮类成分进行含量测定，该方法专属性强、重复性好、稳定、可控，可作为黄芪药材质量控制的方法。

英文摘要：

OBJECTIVE To establish an UPLC method for simultaneous detellnination of six flavonoid active components （ campanulin, ononin, quercetin, calycosin, kaempferol, and formononetin ） of the roots of Astragalus membranaceus （Fisch.） Bge. vat. mongholicus （Bge.） Hsiao collected from the main producing areas （Gansu Province, Shaanxi Province, and Inner Mongolia）. METHODS Separation was performed at 30℃ on an ACQUITY UPLC BEH C18 column （2. 1 mm x 100 mm,1.7 μm） with a gradient elution system of water containing 0. 1% formic acid （mobile phase A） and acetonitrile - isopropanol （7：3 ） containing 0. 1% formic acid （ mobile phase B）. The flow rate was 0. 25 mL ·min - 1. The detection wavelength was 254 nm, and the sample injection volume was 2μL. RESULTS The six flavonoid active components showed good linearity in the selected concentration ranges （ r ≥ 0.999） ,with average recoveries of 99.60% -101.2% （n =6） and RSDs of 1.2% -2.0%. CONCLUSION This study for the first time establishes an UPLC method for simultaneous determination of six flavonoid active components （ campanulin, ononin, quercetin, calycosin, kaempferol, and formononetin ） of the roots of Astragalus membranaceus （ Fisch. ） Bge. var. mongholicus （ Bge. ） Hsiao. This method is specific, reproducible, controllable, and can be used for the quality control of Radix Astragali.