中文摘要：

目的：建立UPLC法同时测定大黄中大黄素、大黄酸、大黄酚、大黄素甲醚、芦荟大黄素、番泻苷A、番泻苷B、土大黄苷8个成分的含量。方法：采用ACQUITY BEH C18色谱柱（2.1 mm×50 mm,1.7μm）,以乙腈（A）-0.1%甲酸水溶液（B）为流动相,梯度洗脱（0-1 min,10%B→15%B;1-2 min,15%B→20%B;2-3 min,20%B→30%B;3-4 min,30%B→40%B;4-5 min,40%B→60%B;5-6 min,60%B→65%B;6-7 min,65%B→70%B;7-8 min,70%B→80%B;8-9 min,80%B→85%B）,流速0.3 mL·min-1,柱温35℃,254 nm处检测大黄素、大黄酸、大黄酚、大黄素甲醚、芦荟大黄素,320 nm处检测番泻苷A、B和土大黄苷,对测定结果进行主成分分析。结果：大黄素、大黄酸、大黄酚、大黄素甲醚、芦荟大黄素、番泻苷A、番泻苷B、土大黄苷浓度在一定范围内,与峰面积积分值线性关系良好（r≥0.9996）,方法的精密度RSD为0.51%-0.97%,重复性RSD为0.64%-1.3%,稳定性RSD为0.72%-1.6%,平均加样回收率为96.5%-104.2%。主成分分析结果表明正品大黄与伪品可以明显分开,野生品与栽培品内在质量存在差异。结论：所建立的方法能同时测定大黄中8个成分的含量,可作为大黄药材与伪品的区分和质量控制的参考方法。

英文摘要：

Objective：To develop a ultraperformance liquid chromatography（ UPLC） method to determine the content of emodin,rhein,chrysophanol,physcion,aloeemodin,sennoside A,sennoside B and rhaponticin simultaneously in Radix et Rhizoma Rhei. Methods：The analysis was achieved with an ACQUITY BEH C18 analytical column（ 2.1 mm ×50 mm,1.7 μm） by gradient elution of 0. 1%（ v/v） formic acid in water and acetonitrile：0-1 min,10% B→15% B; 12 min,15% B→20% B; 23 min,20% B→30% B; 34 min,30% B→40% B; 45 min,40% B→60% B; 56 min,60% B→65% B; 67 min; 65% B→70% B; 78 min,70% B→80% B; 89 min,80%B→85% B. The flow rate was 0. 3 mL·min1,the column temperature was maintained at 35 ℃ and the detection wavelength was set at 254 nm and 320 nm. Five free anthraquinones were determined at 254 nm,and sennoside A /B and rhaponticin were determined at 320 nm. Principal component analysis（ PCA） was used to analyze the test results. Results：The peak areas and concentrations of emodin,rhein,chrysophanol,physcion,aloeemodin,sennoside A,sennoside B and rhaponticin showed good linear relationship within a certain concentration range（ r ≥0. 9996）; the RSD of precision was 0. 51%0. 97%; the RSD of repeatability was 0. 64%1. 3%,the RSD of stability was 0. 72%1. 6% and the average recoveries were 96. 5%104. 2%. Quality and counterfeit of Radix et Rhizoma Rhei could be clearly separated by PCA and there were differences in intrinsic quality between wild variety and cultivation. Conclusion：UPLC method for simultaneous determination of 8 contents of Radix et Rhizoma Rhei could be used to distinguish between qualified products and counterfeits of Radix et Rhizoma Rhei and used as the reference method for quality control.