中文摘要：

为了优化东北刺人参不定根反应器培养体系,获得较高的人参皂苷含量,以东北刺人参无菌苗诱导得到的不定根为试验材料,研究生物反应器不定根培养过程中,蔗糖浓度、生长素IBA浓度、接种量和通气量对皂苷积累的影响。比较3种培养方式发现,生物反应器培养比振荡培养和固体培养更有利于不定根中皂苷积累。在生物反应器培养过程中,随着蔗糖浓度的增加,皂苷含量明显提高,在蔗糖质量浓度为50 g/L达到最大;IBA质量浓度为3 mg/L时最利于皂苷积累;接种量为5 g/L时不定根中皂苷含量和生产量最多,而接种量超过5 g/L不利于皂苷积累;通气量75 m L/min时,皂苷含量达到最高。因此,利用生物反应器培养不定根来生产皂苷,为资源短缺的东北刺人参产品开发提供了新方法,对今后的东北刺人参产品生产具有重要意义。

英文摘要：

As a perennial woody medicine plant,Oplopanax elatus has high pharmaceutical values. The saponin is the one of the main effective compounds in O. elatus. However,the production of O. elatus has been restricted by the shortage of the plant resources. Therefore,the present study used adventitious roots derived from sterilized seedlings as the experimental materials to mass-production of saponins by adventitious root culture in bioreactor systems. The adventitious root growth and saponin content among the different culture systems（ bioreactor culture,shake culture,and solid culture） were compared. The results showed that the bioreactor culture was more beneficial for adventitious root biomass（fresh and dry weight） and saponin accumulation. During the bioreactor culture,sucrose concentrations in the culture medium crucially affected the adventitious root growth and saponin accumulation,and the maximum saponin content of adventitious roots were found at 50 g / L sucrose. IBA concentrations also had an obvious effect on the adventious root culture in bioreactors,and 3 mg / L IBA was the most favorable concentration. In addition,the inoculation density of 5g / L promoted the adventitious root growth and saponin accumulation,while the densities more than 5 g / L had the inhibitory effect. The effect of air volume was also significant,and the maximum adventitious root biomass and saponin content were found at 75 m L / min air volume,while the air volume higher or lower than 75 m L / min restricted adventitious root growth and saponin accumulation. Consequently,the saponin-production of the O. elatus via adventitious root culture using bioreactor systems was feasible and the massive yields of adventitious root and saponins can be obtained by controlling the bioreactor culture conditions. The present study indicated that the bioreactor culture of adventitious roots was an alternative approach to pave the way for the industrial production of O. elatus products.