中文摘要：

东北刺人参是五加科兼具观赏和药用价值的珍贵濒危植物。为了促进东北刺人参产品的开发,在抗炎相关产品生产中以不定根代替短缺的野生植株作为植物材料。该研究利用诱导子未处理和处理的生物反应器培养的东北刺人参不定根水提物处理小鼠腹腔巨噬细胞,通过检测巨噬细胞中一氧化氮（NO）和肿瘤坏死因子-α（TNF-α）及白细胞介素-1β（IL-1β）3种促炎介质的水平,探明不定根的抗炎活性。研究表明：2种东北刺人参不定根提取物中总酚和总黄酮含量有很大差异,诱导子处理的不定根水提物（E 2）中总酚含量是诱导子未处理不定根水提物（E 1）的3.2倍,而总黄酮含量是2.7倍。2种不定根提取物对NO和TNF-α及IL-1β的影响不同,E2对3种促炎介质均有显著的抑制作用,但E1只抑制NO的生成,对TNF-α及IL-1β没有影响,E2的抗炎效果高于E1。因此,今后在进行抗炎相关产品生产中,可利用在生物反应器培养中经诱导子处理的东北刺人参不定根作为原材料。

英文摘要：

Oplopanax elatus Nakai is a plant of Araliaceae with ornamental and medicinal values.To promote the product development of O.elatus,the adventitious roots replaced the field grown plants in the anti-inflammatory production.In the present study,the water extracts of O.elatus adventitious roots,which cultured with the untreated bioreactor（E1）and the treated bioreactor with an elicitor（E2）,was used to treat the peritoneal macrophages of the mice.The anti-inflammatory activity was investigated by measuring the levels of NO,TNF-α,and IL-1β.In the water extracts of the E1 group and the E2 group,the total phenol and total flavonoid contents was different.The total phenol and the flavonoid contents in E2 group were 3.2 fold and 2.7 fold more than that in the E1 group.The effect of the both extracts on the production of NO,TNF-α,and IL-1β was also different,three pro-inflammatory mediators were all inhib-ited by the E2 group,while only NO was inhibited by the E1 group;the anti-inflammatory activity of the E2 group was higher than that of the E1 group.Consequently,bioreactor cultured adventitious roots treated with elicitor can be used in the anti-inflammatory production of O.elatus in the future.