中文摘要：

以狼爪瓦松种子诱导的愈伤组织为材料,研究了光照强度对愈伤组织生物量及黄酮积累的影响。结果表明：光照强度为15-30（μmol·cm-2·s-1）时,有利于狼爪瓦松愈伤组织鲜重和干重的增加;对于狼爪瓦松中酚的积累,光照强度为15μmol·cm-2·s-1）时,其含量（17.4mg/g DW）及生产量（68.53mg/L）最高。而当光照强度达到30（μmol·cm-2·s-1）时,可得到最高的多糖含量（125.8 mg/g DW）和生产量（615.05mg/L）。对于黄酮类物质,光照强度为15（μmol·cm-2·s-1）时,最有利于山奈酚-3-O-芸香糖苷的积累,而槲皮素、山奈素、表儿茶素没食子酸酯和异槲皮苷的积累在光照强度为30（μmol·cm-2·s-1）时最佳;总黄酮的含量（3 816.6mg/g DW）和生产量（18 654.0mg/L）在光照强度30（μmol·cm-2·s-1）时达到最大值。因此,光照强度是狼爪瓦松愈伤组织有效物质积累和生物量增加的影响因素之一,在愈伤组织培养时将光照强度调节为30（μmol·cm-2·s-1）较为适宜。

英文摘要：

In this study, the calluses induced from O. cartilagineus seeds were used as the experimental material to investigate the effects of light intensity on callus biomass and effective compound accumula- tion. The results showed that the light intensity from 15 to 30 μmol ·cm-2 · s- 1 was beneficial to callus biomass accumulation, the fresh and dry weight reached the maximum level. For effective compound accu- mulation, the highest total phenolic content （17.4 mg/g DW） and productivity （68.53 mg/L） were found under 15 μmol · cm-2 · s- 1of light intensity, but the maximum polysaccharide content （125.8 mg/g DW） and productivity （615.05 rag/L） were obtained under 30 μmol · cm-2 · s -1of light intensity. In addition, light intensity also affected flavonoid accumulation, 15 μmol · cm-2 · s-1 of light intensity was the most favorable for kaempferol-3-O-rutinoside accumulation, but the quercetin, kaempferol, epicatechin gallate, and isoquercitrin enhanced under 30 μmol· cm-2 · s-1 of light intensity. The total flavonoid content （3 816.6 mg/g DW） and productivity （18 654.0 mg/L） also reached the maximum under 30 μmol · cm-2 ·s -1 light intensity. Therefore, the light intensity is one of important factors in O. cartilaginous callus culture for production of effective compounds and 30 μmol · cm -2· s-1 is the suitable light intensity.