中文摘要：

应用LA—PCR技术，扩增得到1291 bp的Mucor sp．EIM-10△^15脂肪酸脱氢酶启动子区域的单一产物。对该产物测序，经序列比对分析，发现该序列具有真核启动子序列的基本结构特征，含有TATA盒、CAAT盒、GC盒等元件。根据本中心已构建的质粒PYD15PMCD15和质粒PYGFP，构建启动子功能鉴定阴性质粒PYMCD15，将扩增得到的△^15脂肪酸脱氢酶启动子区域序列连接到PYMCD15中，构建重组表达质粒PYMD15PMCD15，转化Saccharomy cescerevisiae尿嘧啶营养缺陷型INVScl。发酵诱导后GC／MS分析，结果表明△^15脂肪酸脱氢酶启动子区域能够启动Mucor sp．EIM～10△^15脂肪酸脱氢酶基因的表达。

英文摘要：

A 5＇ flanking region DNA of △^15- fatty acid desaturase from Mucor sp. EIM- 10 was specially ampli- fied by LA PCR, which was cloned into pMD- 18 T vector and sequenced. The sequence contained a novel promoter region with TATA box, CAAT box and GC-box like elements in the identical positions common to the eukaryote promoter sequence regions. According to the plasmid of PYD15PMCD15 and PYGFP, the plasmid of PYMCD15 was constructed. The amplified product of promotor region was ligated into the PYMCD15 vector which contained the △^15- fatty acid desaturase gene to construct the expression plasmid PYMD15PMCD15. The recombined plasmid was transferred into Saccharomyces cerevisiae strain INVScl. The GC/MS analysis on the profile of the total fatty acid demonstrated that the novel peak for product ALA appeared in the transgenetic yeast compared with the control yeast containing the original vector PYMCD15.