中文摘要：

通过在薄膜金电极上修饰具有较高电子转移效率的氧化还原聚合物,并固定相应的酶,制备了一种可用于检测低浓度葡萄糖的生物传感器。研究了利用戊二醛交联和光聚合两种酶固定化方法制备出的传感器对检测低浓度葡萄糖的动态响应特性影响。结果表明;利用戊二醛交联方法制备的传感器灵敏度更高,且具有更好的稳定性。在0～500μM浓度范围内,传感器对葡萄糖干法电流响应灵敏度为35.4 nAμM^-1cm^-2,检测限为0.5μM,响应时间30 s,对50μM葡萄糖的精密度为4.4%。

英文摘要：

A biosensor for the determination of low concentration glucose was prepared by immobilizing enzyme on the thinfilm Au electrode surface modified redox polymer. The analytical characteristics were compared by different immobilized enzyme methods including photo polymerization and glutaraldehyde cross-linking. The biosensor fabricated by glutaraldehyde cross-linking showed higher sensitivity and better stability. Glucose biosensor exhibited analytic sensitivity of 35.4 nAμM^-1cm^-2 in the relevant concentration range of 0 - 500 μLM at the response time 30 s. The limit of detection for glucose was 0.5μM. The coefficient of variation was 4.4%.