中文摘要：

在塑料基片上制备了金薄膜的两电极系统的生物传感器。集成化的Ag｜AgCl参比电极采用丝网印刷在薄膜电极上。以聚乙二醇二缩水甘油醚（PEGDGE）为交联剂，将辣根过氧化酶（HRP）和谷氨酸氧化酶（GLOD）固定到聚乙烯吡啶与2，2＇-双吡啶锇形成的氧化还原复合物中，滴在电极表面形成传感器的工作电极。实验结果表明，该生物传感器在谷氨酸溶液的浓度为1．0×10^-6～4．0×10^-4moL／L的范围内，具有很好的线性关系（r=0．9998，n=9），灵敏度为37．5mA（moL／L）^-1cm^-2；谷氨酸检出限为1.0×10^-6mol／L；传感器响应时间为10s且有良好的一致性和稳定性，使用一个月后仍能保持其初始活性的90％。该传感器可以用于食品工业中的谷氨酸的快速检测和在线监测等。

英文摘要：

A two-electrode configuration has been fabricated by depositing gold film on a polyethylene terephthalate（PET） substrate. The integrated reference electrode was obtained by screen-printing commercial silver and silver chloride paste on the gold electrode. The working electrode was modified by drop-coating a redox polymer formed of poly （4-vinylpyrodine） complexed with （osmium （2,2＇-bipyrridine）2. The bienzyme, horseradish peroxidase （HRP） and glutamate oxidase （GLOx）, were immobilized in the redox matrix by the （poly（ethylene glycol） diglycidyl ether（PEGDGE） crosslinker. The optimized biosensor were characterized by a sensitivity of 37.5 mA（mol/L）^-1cm^-2, a detection limit of 1.0×10^-6 mol/L, a response time of less than 10 s, and a correlation coefficient during a concentration ranged from 1.0×10^-6 mol/L to 1.0×10^-4 mol/L is 0.9998 （n = 9）. The biosensor exhibits excellent reproducibility and stability, retaining more than 90% of its original activity over a period of one month. The simple fabrication of the biosensor can be used to determinate L-glutamate rapidly in food industry and clinical diagnose.