中文摘要：

将锇氧化还原聚合物与辣根过氧化酶共价交联修饰薄膜金电极,使用戊二醛交联固定葡萄糖氧化酶制得葡萄糖传感器,对传感器的制备条件和其对低浓度葡萄糖的响应特性进行了研究。在-0.1V（vs.Ag｜AgCl）电位下,该传感器的背景电流小于1nA,检出限为1μM,小于400μM范围内灵敏度为2900nAμM^-1（相关系数R=0.998）,且能够消除抗坏血酸对葡萄糖测定的干扰,实现了低浓度葡萄糖的测定,为实现血糖的无创监测奠定了基础。

英文摘要：

The fabrication and analytical characterization of an osmium redox polymer modified glucose biosensor for measurement of low concentration glucose is described. The thin film gold electrode was modified with osmium redox polymer wired horseradish peroxidase and immobilization of the glucose oxidase was then operated using glutaraldehyde to build the glucose sensor. The developed sensor characterized a low background current less than 1 nA and a detection limit of 1 μM, as well as a high sensitivity of 2900 nA μM^-1 in the linear range up to 400 μM at low applied potential of -0. 1 V （vs. Ag｜ AgCl）. The oxidation interference of ascorbic acid for the determination of glucose can be prevented due to the low applied potential. These results testified the feasibility of the sensor for measurement of low concentration glucose and have significance for the development of noninvasive glucose monitoring system for the control of diabetes.