中文摘要：

目的评估大骨节病患者软骨细胞的线粒体功能及其与年龄的关系。方法关节软骨细胞体外培养,分光光度法测定呼吸链复合体活性,化学发光法检测细胞内ATP,流式细胞仪检测线粒体膜电位和细胞内活性氧含量,线性回归分析线粒体功能与年龄的相关性。结果大骨节病线粒体呼吸链复合体Ⅱ（P=0.001）、Ⅲ（P=0.005）和Ⅳ（P=0.005）活性降低,柠檬酸合酶活性升高（P=0.04）,细胞内ATP含量减少（P=0.001）,线粒体膜电位下降（P=0.001）,细胞内活性氧含量增加（P〈0.001）;线粒体功能与年龄在大骨节病和正常对照组均无显著相关性。结论大骨节病软骨细胞存在线粒体功能障碍,线粒体功能与年龄间无显著相关性。

英文摘要：

Objective To evaluate the mitochondrial function of chondrocytes in Kashin-Beck disease （KBD） patients and its correlation with age.Methods Mitochondrial function was evaluated by analyzing the activities of respiratory chain enzyme complexes and citrate synthase （CS）,intracellular adenosine triphosphate （ATP）contents, changes in mitochondrial membrane potential （ΔΨm）,as well as the intracellular reactive oxygen species （ROS） contents.Correlation of mitodhondrial function and age was analyzed with linear regression.Results Activities of complexes Ⅱ （P = 0.001 ）,Ⅲ （P = 0.005 ）and Ⅳ （P = 0.005 ）were reduced in KBD articular chondrocytes compared with cells from normal controls.However,the mitochondrial mass was increased in KBD samples. Cultured KBD chondrocytes had reduced cellular ATP level （P = 0.001 ）and mitochondrial membrane potential （P =0.001）,but increased citrate synthase activity （P =0.04）and intracellular ROS level （P 〈0.001）.We found no correlation between mitochondrial function and donor age either in normal or in KBD chondrocytes. Conclusion Mitochondrial function is altered in articular chondrocytes of KBD,and no correlation was found between mitochondrial function and donor age.