中文摘要：

目的：探讨蛋白酶激活受体1和2（PAR1和PAR2）在结肠癌细胞株SW620和SW480细胞的表达及其作用。方法：采用免疫细胞化学染色及RT-PCR法检测SW620和SW480细胞有无PAR1和PAR2蛋白及其mRNA的表达；利用各自不同的激动剂、拮抗剂及相关抗体，观察对细胞迁移和增殖能力的影响以及对细胞分泌白细胞介素8（IL-8）的影响。结果：结肠癌细胞株SW620和SW480均有PAR1和PAR2mRNA的表达，但仅见SW620细胞表面具有PAR2蛋白的高表达，PAR1蛋白表达不明显；SW480细胞表面既无PAR1也无PAR2蛋白的表达。SW620细胞的迁移能力明显高于SW480细胞，PAR1和PAR2的激动剂以及因子Ⅶa均可促进SW620细胞的增殖、迁移和IL-8的分泌；PAR2拮抗剂对细胞迁移起抑制作用，抗PAR2抗体对细胞的增殖和迁移均有干预作用。结论：蛋白酶激活受体2在结肠癌细胞表面大量表达，可增强细胞的增殖和迁移能力及IL-8的分泌，表明与肿瘤的转移能力及恶性程度密切相关。

英文摘要：

Objective： To investigate the expression and functions of protease-activated receptor 1 and 2 （PAR1 and PAR2） on colon tumor cell lines, SW620 and SW480 cells. Methods： The expression of PAR1 and PAR2 on SW620 and SW480 cells was investigated through immunocytochemistry and RT-PCR at antigen and mRNA level. The cell growth and proliferation rate, migration ability as well as interleukin 8 （IL-8） induction levels were evaluated under the conditions with different agonists, antagonist and antibod- ies of PAR1 and PAR2. Results： Both SW620 and SW480 cells could express PAR1 and PAR2 mRNA. PAR2 antigen, but not PAR1 antigen, was strongly shown on SW620 cell surface. Neither PAR1 nor PAR2 antigen was seen on SW480 cell surface. The natural migratory potential of SW620 cells was higher than that of SW480 cells. The ability of proliferation and migration in SW620 cells and induction of IL-8 protein in SW620 cells were obviously increased with PAR1, PAR2 agonists and factor Via. PAR2 antagonist inhib- ited cell migration, and anti-PAR2 antibody decreased cell proliferation and migration. Conclusion： Protease-activated receptors 2 were strongly expressed on colon cancer cells, enhancing cell proliferation and migration as well as IL-8 production, which was closely associated with cancer metastasis and the malignant behavior.