中文摘要：

为研究NaF、NaBr、NaI对酵母朊病毒[PSI^＋]形成的影响，利用表达融合蛋白GFP—Sup35p酵母朊病毒模型，借助半变性琼脂糖凝胶电泳技术，结合免疫印迹方法在蛋白水平上定量分析NaF、NaBr、NaI对酵母朊病毒[PSI^＋]形成的影响。结果显示，在细胞表型方面NaF、NaBr诱导出酵母朊病毒[PSI^＋]，且所需浓度分别为0．02、1．0mol／L；NaI在浓度为0．25mol／L可以诱导出[PSI^＋]经盐酸胍治愈后的酵母朊病毒[psi^＋]。在蛋白水平，NaF、NaBr、Nal盐作用后的[psi-]细胞内Sup35p并没有形成聚集体。

英文摘要：

In order to study the impact of NaF, NaBr, NaI on the formation of yeast prion [ PSI＋ ], the expression fusion protein GFP-Sup35p yeast priori model was used with the aid of semi-denaturing agarose gel dectrophoresis combined with Western blot method to quantitatively analyse the impact of NaF, NaBr, NaI on the formation of yeast prion [PSI＋ ] at the protein level. The results showed that in the aspect of cell phenotype, NaF, NaBr induced out yeast prion [ PSI＋ ] , and the desired concentrations were at 0.02 mol/L, 1.0 mol/L; NaI at concentration of 0. 25mol/L can induce [ PSI＋] by guanidine hydrochloride cure yeast prion[psi-]. At the protein level, NaF, NaBr, NaI salt after [psi- ] intracellular Sup35p did not form aggregates.