中文摘要：

目的：研究夜香树（CN）叶正丁醇提取物中流份C6、C7对人胃癌细胞SGC7901增殖和凋亡的影响。方法：采用不同比例的氯仿-甲醇（1∶9、1∶7）对CN叶正丁醇部位梯度洗脱,得到流份C6和C7。将SGC7901细胞分为空白对照组和C6、C7组。分别用0（空白对照）、5、10、20、40、80μg/ml的C6、C7培养SGC7901细胞72 h后采用MTT法检测其对细胞的增殖抑制作用,计算增殖抑制率、半数抑制浓度（IC50）;用10μg/ml C6、C7培养SGC7901细胞72 h后,采用集落形成法检测其对细胞的集落形成能力的影响,计算集落形成率;瑞氏-姬姆萨混染法、Hoechst 33258/碘化丙啶（PI）染色法观察细胞形态学变化。结果：MTT结果显示,流份C6、C7对SGC7901细胞增殖有不同程度的抑制作用,抑制率分别为22.1%~80.0%、19.6%~79.7%,IC50分别16.4、18.05μg/ml。与空白对照组比较,C6和C7组细胞的集落形成率降低,差异有统计学意义（P〈0.05）;给药组细胞凋亡细胞、死细胞更多。结论：CN叶正丁醇提取物中流份C6、C7可显著抑制SGC7901细胞增殖并诱导其凋亡。

英文摘要：

OBJECTIVE：To study the effects of flow components C6 and C7 in n-butyl alcohol extract from the leaves of Cestrum Nocturnum（CN）on the proliferation and apoptosis of human gastric cancer cell SGC7901. METHODS：C6 and C7 were obtained by using different ratio of chloroform and methanol（1 ∶ 9,1 ∶ 7）to the gradient elution of CN leaves. After cultured with 0（blank control）,5,10,20,40,80 μg/ml C6 and C7 for 72 h,inhibitory effect of C6 and C7 on the proliferation of SGC7901 was determined by MTT assay. Inhibitory rate and IC50 were calculated. After SGC7901 were cultured with 10 μg/ml C6 and C7 for 72 h,colony formation assay was utilized to detect the effects of C6 and C7 on the cell colony formation,and the rate of colony formation was calculated. In addition,Wright/Giemsa and Hoechst33258/PI staining assay were used to observe the change of cytomorphology. RESULTS：MTT showed that C6 and C7 had inhibitory effect on the proliferation of SGC7901 to different extent;inhibitory rates were 22.1%-80.0% and 19.6%-79.7%,and IC50 were 16.4,18.05 μ g/ml,respectively. Compared with blank control group,colony formation rate of C6 and C7 group decreased,with statistical significance（P〈0.05）. The number of apoptotic cells was more in treatment group than in other groups. CONCLUSIONS：Flow components C6 and C7 in n-butyl alcohol extract from the leaves of CN can inhibit the proliferation of SGC7901 cells and induce the apoptosis of them.