中文摘要：

目的：建立美味猕猴桃根与茎中熊果酸的含量测定方法。方法：采用HPLC法，色谱柱为Thermo Hypersil BDSC148柱（4．6mm×250mm，5μm）；流动相为甲醇-0．2％醋酸铵（85：15）；流速为0．8ml／min；柱温为25℃；检测波长为210nm。结果：熊果酸在0．204～2．04μg范围内，峰面积与进样量呈良好的线性关系，r=0．9998；平均加样回收率为101．88％，RSD为1．90％（n=6）。结论：该方法简便可行，重复性好，结果准确，可作为美味猕猴桃中熊果酸的含量测定方法。

英文摘要：

Objective： To establish a determination method for the ursolic acid in the stems and toots of Acitinidia deliciosa C. F. liang et A. R. Ferguson. Methods： The way of HPLC was used. Chromatographic column was Thermo Hypersil BDS Cls （4.6mm×250mm,5μm） and the mobile phase was a mixture of methanol-0.2% ammonium acetate （85： 15） with the flow rate at 0.8ml·min^-1. Column temperature was at 25℃and the detector was 210nm. Results： When ursolic acid was in the rang of 2.91-29.1μg, the natural logarithm （Y） of peak area and the natural logarithm （X） of sample size showed a satisfactory linear relationship r= 0.9998;the average recovery of application of sample was 101.88% and RSD was 1.90% （n = 6）. Conclusion： The method is easy which has a good repeatability and an accurate result. It can be the method for the determination for ursolic acid in Fortune euonymus stem.