中文摘要：

目的：观察受体酪氨酸激酶c-kit及其配体干细胞因子（stem cell factor，SCF）在人正常肝、肝硬化、癌旁肝及肝癌组织中的共表达。方法：用免疫组化／免疫荧光染色方法和组织芯片检测受体酪氨酸激酶c-kit及其配体在166例肝癌和配对癌旁肝、16例肝硬化及10例正常肝组织中的表达差异及其共表达状况。结果：免疫组化检测c-kit在肝癌、癌旁肝、硬化肝和正常肝组织中的阳性率分别为47．59％，95．78％，93．75％和100％，其中强阳性（≥＋＋）率分别为27．71％，77．71％，87．53％和100％，c-kit蛋白在肝癌组织中明显低表达；而SCF在肝癌、癌旁肝、硬化肝和正常肝组织中的阳性率分别为68．07％，100％，100％和100％，其中强阳性（≥＋＋）率分别为35．54％，94．58％，93．75％和100％，SCF在肝癌组织中也呈现低表达；但SCF在各种组织中的表达均高于c-kit。免疫荧光检测受体酪氨酸激酶c-kit及其配体SCF在各种组织中均有不同程度的共表达，且均定位于肝癌细胞和肝细胞的细胞质中，其中在肝癌、癌旁肝、硬化肝和正常肝组织中的共表达率分别为51．20％，100％，100％和100％，而肝癌组织的强阳性共表达（≥＋＋）率仅为25．30％，其他组织均高于95％。结论：与正常肝、硬化肝、癌旁肝组织相比，肝癌组织中c-kit和SCF均呈低表达，但SCF在相应组织中的表达稍高于c-kit；正常肝、硬化肝、癌旁肝组织中c-kit／SCF共表达远高于肝癌组织。

英文摘要：

Objectives： To evaluate the co-expression of the receptor tyrosine kinase c-kit and its ligand stem cell factor （SCF） in tissues from human normal liver, cirrhotic liver, noncancerous liver, and hepatocellular carcinoma （HCC） . Methods： The differential expression patterns and co-expression of c-kit and SCF were examined by immunochemical and immunofluorescent method on a tissue microarray, including samples of 10 human normal livers, 16 cirrhotic livers, 166 HCC, and matched adjacent noncancerous liver tissues. Results： Immunochemical analysis showed that positive rates of c-kit staining were 47. 59%, 95. 78%,93. 75%, and 100% in hepatocellular carcinoma, adjacent noncancerous liver, cirrhotic liver, and all of human normal liver tissues, respectively. The corresponding strong positive（≥＋＋） ratios were 27.7%, 77.7%, 87.5%, and 100.0% ,respectively. The positive rates of SCF were 68.1%, 100%, 100 %, and 100% in HCC, the adjacent noncancerous liver, cirrhotic liver, and human normal liver tissues, respectively. The corresponding strong positive（≥ ＋ ＋） ratios were 35.54 %, 94.58 %, 93.75 %, and 100%, respectively. The expression of SCF was lower in HCC. But the expression of SCF was higher than c-kit in all tissues. Immunofluorescent analysis showed co-expression of c-kit and SCF in various tissues at different degrees. They were localized in the cytoplasm of HCC and normal liver. The co expression rates were 51.20% ,100% ,100%, and 100% in HCC, the adjacent noncancerous liver, cirrhotic liver,and human normal livor tissues, respectively. The strong positive（≥＋ ＋） ratio of co expression was only 25.30 % in HCC and more than 95 % in other three tissues. Conclusion： The expressions of c-kit and its ligand SCF are lower in HCC compared with normal liver, cirrhotic livers, and adjacent noncancerous liver tissues. SCF has slightly higher expression than c-kit in the corresponding tissues. Co-expressions of c-kit and SCF in normal liver, cirrhotic livers, and adjacent noncanc