中文摘要：

目的制备局灶性脑缺血再灌注损伤大鼠模型，并观察大脑皮质细胞色素C（CytC）与Bax蛋白表达的变化。方法采用随机数字表法将22只雄性SD大鼠分为正常组（6只）、假手术组（6只）和模型组（10只）。采用线栓法闭塞大脑中动脉2h后进行再灌注3d，制备局灶性脑缺血再灌注损伤模型。采用神经缺失评分观察大鼠的行为学表现；TTC染色检查脑组织梗死情况；HE染色观察大鼠脑组织形态结构；Western blot技术检测大鼠大脑皮质CytC与Bax蛋白的表达。结果假手术组大鼠无神经功能障碍表现，脑组织未见梗死灶，脑组织神经细胞形态规则；大脑皮质CytC与Bax蛋白的相对表达量，与正常组相比差异无统计学意义（P〉0．05）。与假手术组相比，模型组大鼠出现神经功能障碍，左侧半球可见梗死灶，梗死侧脑组织形态学观察可见神经细胞大量坏死脱落，胞质呈空泡变性、疏松，胞核浓缩深染；大脑皮质CytC与Bax蛋白表达量明显增加（P〈O．05）。结论大脑中动脉闭塞2h后进行再灌注3d可造成脑缺血再灌注损伤，可能与大脑皮质CytC与Bax蛋白的表达增加有关。

英文摘要：

Objective To establish a rat model of focal ischaemia-reperfusion injury, and to ob- serve the changes in cytochrome C and Bax protein expression in cerebral cortex. Methods Twenty-two male SD rats were randomly divided into three groups： normal group（n= 6）, sham- operated group group （n= 6） and model group （n= 10）. Focal cerebral ischemia-reperfusion injury was induced by middle cerebral artery occlusion for 2 hours and reperfusion for 3 days. Behavioral performance, brain infarction,brain morphology and cortical expression of cytochrome C and Bax protein were measured using Neurologic Deficit Score, TTC staining, HE staining and Western blotting, respectively. Results Rats in sham-operated group showed regular neuronal cell mor- phology without neurological dysfunction and brain infarction. There were no significant differ- ences in cortical expression of cytochrome C and Bax protein between sham-operated group and normal group （P〉0.05）. Compared with sham-operated group, cortical expression of cytochrome C and Bax protein significantly increased in model group （P〈0.05）. In addition, rats in model group showed neurological dysfunction, left hemisphere infarction, neuronal cell necrosis, cyto-plasmic vacuolar degeneration,and deeply stained nucleus. Conclusion Middle cerebral artery oc- clusion （MCAO） for 2 hours and reperfusion for 3 days can induce cerebral ischemia-reperfusion injury,which may be correlated with the increase in the expression of cytochrome C and Bax pro- teln.