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中文摘要：
为了研究鸡TGF-β2基因启动子区的SNP及其对启动子功能元件的影响,采用PCR-SSCP方法对16个鸡种的TGF-β2启动子区的554~824 bp（即3号染色体的19 433 476~19 433 746 bp）进行基因分型,经测序,共检测到3个有效的SNPs位点,分别是679位点的C/G,699位点的G/A,774位点的T/C。其中大骨鸡和太湖鸡在3个位点均检测到SNP存在,AA鸡在3个位点都没有发现SNPs。分别利用生物信息学软件JASPAR和Meth Primer预测TGF-β2基因启动子区转录因子结合位点和Cp G岛,发现SNP位点可改变转录因子结合位点且位于Cp G岛上,表明TGF-β2启动子区SNPs可能通过不同方式影响基因表达调控。
英文摘要：
To study the SNP in TGF-β2 gene promoter and its effect on the promoter functional elements,genotyping was used by PCR-SSCP in the region of 554-824 bp（19 433 476-19 433 746 bp in Chromosome 3） of the promoter of TGF-β2 gene among 16 chicken breeds. After the direct sequencing,we found three SNP loci totally in all breeds：the C/G in 679 loci,the G/A in 699 loci and the T/C in 774 loci. Three SNPs were detected in the Big-bone chicken and Taihu chicken,but no SNP was found in AA chicken. Moreover,we analyzed the transcription factor binding sites and Cp G island by JASPAR and Meth Primer software respectively in TGF-β2 gene promoter,and found that SNPs changed the transcription factor binding sites and the Cp G islands. The results suggested that SNPs in TGF-β2promoter played an important role in the regulation of gene expression through different ways.
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