中文摘要：

以朝鲜淫羊藿幼叶、叶柄和茎尖为外植体,研究了朝鲜淫羊藿愈伤组织诱导的适宜条件,以期为工厂化育苗提供科学根据。结果表明：朝鲜淫羊藿愈伤组织诱导最佳外植体为茎尖,接种于培养基MS＋2,4-D 2.0mg/L＋6-BA 0.5mg/L＋NAA 1.0mg/L中,于25℃黑暗条件下培养1周后转入光照强度2 000lx条件下,愈伤组织诱导效果最佳。

英文摘要：

Taking the young leaf,stem apex and petiolesuitable of Epimedium koreanum Nakai as explants,the condition of its callus induction was studied,in order to provide scientific basis for large scale production of tissue culture.The results showed that the stem apex was the best production material of tissue culture.The optimum medium for callus induction was MS＋2,4-D 2.0 mg/L＋6-BA 0.5 mg/L＋NAA 1.0 mg/L.In the temperature of 25℃,the induction effect was good under the cultured conditions of dark,and taken out,then cultured in the light intensity 2 000 lx.