中文摘要：

目的建立HPLC法同时测定淫羊藿药材中朝藿定A、朝藿定B、朝藿定C、淫羊藿苷、宝藿苷I等8种黄酮类成分含量的方法。方法色谱柱为Agilent Zorbax SB-C18柱（250 mm×4.6 mm,5μm）,流动相为乙腈-水梯度洗脱,检测波长为270 nm,流速为1.0 m L·min-1,柱温为30℃。结果朝藿定A、朝藿定B、朝藿定C、淫羊藿苷、宝藿苷I、朝藿苷丙、epimedokoreanoside I、朝藿苷乙等8种黄酮类成分的线性关系良好,线性范围分别为2.0~20.2、1.0~57.2、3.0~132.0、1.0~50.0、1.0~45.2、0.4~15.0、0.6~25.8、0.2~52.7 mg·L-1;平均回收率分别为98.3%、96.7%、97.2%、96.9%、95.5%、98.5%、98.2%、98.5%,RSD分别为1.0%、0.7%、0.9%、0.6%、0.2%、1.1%、1.4%、0.3%（n=6）。结论该方法可作为淫羊藿药材含量测定的方法。对收集的104批市售的淫羊藿药材中淫羊藿苷含量进行了测定,淫羊藿苷含量质量分数为0.01%~0.17%,大部分不符合《中华人民共和国药典》标准。

英文摘要：

Objective To develop a method for the quantitative analysis of epimedin A,epimedin B,epimedin C,icariin,baohuoside I etc.,totally 8 flavonoids in Epimedium.Methods The analysis was performed by using a Zorbax SB-C18 analytical column（250 mm × 4.6 mm,5 μm） at gradient elution of acetonitrile and water with diodearray detection（270 nm）,the flow rate was 1.0 m L·min-1,the column temperature was maintained at 30 ±0.15 ℃.Results All calibration curves showed good linearity（r 〉 0.999 4） within test ranges.The recoveries were95.5%-98.5% and the RSD for intra-day was less than 3.0% at six levels.Conclusions This method is reliable,simple and reproducible.It can be used for the determination of Epimedium.The contents of icariin in 104 samples were between 0.01%-0.17% and much lower than the Pharmacopoeia standards.The quality of commercially available Epimedium is not optimistic,most of them are unqualified.