中文摘要：

目的建立HPLC法测定江枳壳药材中柚皮素和橙皮素含量的方法。方法采用Diamonsil C18色谱柱（150 mm×4.6 mm,5μm）,以乙腈-水（磷酸调pH值2-3）=29：71为流动相,流速1.0 m L·min^-1,柱温25℃,检测波长283 nm。结果柚皮素和橙皮素进样量分别在0.065 8-0.658μg和0.021 2-0.212μg范围内呈良好的线性关系,相关系数均为0.999 8;柚皮素和橙皮素平均回收率（n=6）分别为102.2%和101.7%,其RSD值分别为1.7%和2.3%。江枳壳中柚皮素和橙皮素含量分别在0.09‰-0.35‰和0‰-0.11‰范围内。结论柚皮素和橙皮素的含量随采收期的推迟而降低。本法简便、准确、可靠,可用于枳壳药材中柚皮素和橙皮素的含量测定。

英文摘要：

Objective：To establish a method for the quantification of naringenin and hesperetin in fructus aurantii by HPLC.Methods：The experimental conditions list as follow diamonsil C18（150 mm×4.6 mm,5 μm） column,mobile phase of acetonitrile-phosphoric acid water（29：71,pH = 2~3）,25℃,flow rate of 1.0 m L·min~（-1）,detection wavelength of 283 nm.Results：The method had good linear relationship within the range of 0.065 8-0.658 μg（r = 0.999 8） for naringenin and 0.021 2~0.212 μg（r = 0.999 8） for hesperetin.The average recoveries（n = 6） were 102.2%（RSD = 1.7%） for naringenin and 101.7%（RSD = 2.3%） for hesperetin.The contents in Jiangxi fructus aurantii were 0.09‰-0.35‰ for naringenin and 0‰~0.11‰ for hesperetin respectively.Conclusion：The contents of naringenin and hesperetin were low at late harvest time.This method is convenient,accurate and reliable for the quantification of naringenin and hesperetin in fructus aurantii.