中文摘要：

目的：建立HPLC法同时测定化橘红药材中水合橘皮内酯、橘皮内酯、马尔敏和葡萄内酯的含量。方法：采用Diamonsil C18色谱柱（250 mm×4.6 mm,5μm）,以甲醇（A）-水（B）为流动相,二元梯度洗脱（0～6 min,49%A;6～15 min,49%A→75%A;15～26 min,75%A→100%A;26～30 min,100%A;30～32 min,100%A→49%A;32～35 min,49%A）,流速1.0 mL·min^-1,检测波长324 nm,柱温30℃。结果：水合橘皮内酯、橘皮内酯、马尔敏和葡萄内酯的进样量分别在0.378 0～2.268μg（r=0.999 7）、0.088 56～0.531 4μg（r=0.999 7）、0.089 58～0.537 5μg（r=0.999 8）和0.053 38～0.320 3μg（r=0.999 9）范围内呈良好的线性关系;平均回收率（n=6）分别为100.6%、101.5%、99.68%、98.93%,RSD为2.9%、2.7%、2.2%、2.3%。12批化橘红样品中水合橘皮内酯、橘皮内酯、马尔敏和葡萄内酯含量测定结果分别为0.123%～0.452%、0.036%～0.094%、0～0.090%、0～0.035%。结论：经方法学验证,本法可用于化橘红药材中水合橘皮内酯、橘皮内酯、马尔敏和葡萄内酯的含量测定。

英文摘要：

Objective： To develop an HPLC method for simultaneous determination of meranzin hydrate,meranzin,marmin and aurapten in Citri Grandis Exocarpium. Methods： The HPLC system consisted of a Diamonsil C18column（ 250 mm × 4. 6 mm,5 μm） and the mobile phase consisted of methanol（ A）-water（ B） in gradient mode（ 0-6min,49% A; 6-15 min,49% A→75% A; 15-26 min,75% A→100% A; 26-30 min,100% A; 30-32 min,100%A→49% A; 32-35 min,49% A） at the flow rate of 1. 0 mL·min^-1. The detection wavelength was 324 nm and the column temperature was 30 ℃. Results： The contents of meranzin hydrate,meranzin,marmin and aurapten had good linear relationship in the ranges of 0. 378 0-2. 268 μg（ r = 0. 999 7）,0. 088 56-0. 531 4 μg（ r = 0. 999 7）,0. 089 58-0. 537 5 μg（ r = 0. 999 8） and 0. 053 38-0. 320 3 μg（ r = 0. 999 9） respectively,and the average recoveries（ n = 6） of four components were 100. 6% with RSD of 2. 9%,101. 5% with RSD of 2. 7%,99. 68% with RSD of 2. 2% and 98. 93% with RSD of 2. 3% respectively. The contents in 12 samples of Citri Grandis Exocarpium were 0. 123%-0. 452% for meranzin hydrate,0. 036%-0. 094% for meranzin,0-0. 090% for marmin,and 0-0. 035% for aurapten. Conclusion： According to the methodology validation,this method can be applied to the determination of meranzin hydrate,meranzin,marmin and aurapten in Citri Grandis Exocarpium.