中文摘要：

目的：研究枳实及其主要活性成分促进脾虚模型大鼠胃肠运动的机制.方法：将170只大鼠随机分为空白组（10只）和造模组（160只）,造模组大鼠以苦寒泻下＋饥饱失常法复制脾虚模型.造模后,将大鼠随机分为模型组,柚皮苷低、中、高剂量组（3.267、6.535、13.070 mg/mL）,新橙皮苷低、中、高剂量组（3.865、7.730、15.460 mg/mL）,辛弗林低、中、高剂量组（0.252、0.504、1.008 mg/mL）,3个单体成分（即柚-新-辛）低、中、高剂量配伍组和枳实水煎液低、中、高剂量组（0.104、0.208、0.416 g/mL,以生药计）,每天ig给药1次,10 mL/kg,连续7 d.末次给药后,检测大鼠血清胃泌素（GAS）和血浆乙酰胆碱（ACh）、胃动素（MTL）、P物质（SP）、血管活性肠肽（VIP）水平.结果：与空白组比较,模型组大鼠血清GAS水平和血浆ACh、MTL、SP水平降低（P〈0.01）,血浆VIP水平升高（P〈0.05）.与模型组比较,除柚皮苷高剂量组和辛弗林各剂量组大鼠血清GAS水平外,其余各给药组均明显升高（P〈0.05或P〈0.01）;新橙皮苷高剂量组、辛弗林高剂量组和枳实水煎液低剂量组大鼠血浆ACh水平显著升高（P〈0.01）;新橙皮苷中、高剂量组,辛弗林高剂量组,低、中剂量配伍组和枳实水煎液中、高剂量组大鼠血浆MTL水平显著升高（P〈0.05）;柚皮苷低、中剂量组和新橙皮苷各剂量组大鼠血浆SP水平显著升高（P〈0.05或P〈0.01）;柚皮苷低剂量组,辛弗林高剂量组和枳实水煎液低、中剂量组大鼠血浆VIP水平显著降低（P〈0.05）.结论：枳实可能通过促进GAS、ACh、MTL的分泌和抑制VIP的分泌来促进脾虚模型大鼠的胃肠运动;其与3个单体成分对胃肠激素的调节作用有差异.

英文摘要：

OBJECTIVE：To study the mechanism of Aurantii fructus immaturus（AFI）and its main active ingredients in pro-moting gastrointestinal motility of model rats with spleen deficiency. METHODS：170 rats were randomly divided into blank group （10 rats） and modeling group （160 rats）,rats in modeling group was induced models with spleen deficiency by bitter cold diar-rhea＋irregular diet. After modeling, rats were randomly divided into model group, naringin （NA） low-dose, medium-lose, high-dose groups（3.267,6.535,13.070 mg/mL）,neohesperidin（NE）low-dose,medium-lose,high-dose groups（3.865,7.730, 15.460 mg/mL）,synephrine（SY）low-dose,medium-lose,high-dose groups（0.252,0.504,1.008 mg/mL）,compatibility groups with 3 monomer ingredients （NA-NE-SY） low-dose,medium-lose,high-dose and AFI water decoction low-dose,medium-lose, high-dose groups（0.104,0.208,0.416 g/mL,calculated by crude drug）,ig,once a day,10 mL/kg,for 7 d. After the last admin-istration,gastrin （GAS） in serum,and acetylcholine （ACh）,motilin （MTL）,substance P （SP）,vasoactive intestinal peptide （VIP）levels in plasma were detected. RESULTS：Compared with blank group,GAS level in serum and ACh,MTL,SP levels in plasma in model group were reduced（P〈0.01）,VIP level in plasma was increased（P〈0.05）. Compared with model group,ex-cept for the GAS level in serum showed no obvious change in NA high-dose group and SY doses groups,other medicine groups were obviously increased （P〈0.05 or P〈0.01）;the ACh levels in serum were obviouly increased in NE high-dose group,SY high-dose group and AFI water decoction low-dose group（P〈0.01）. MTL levels in plasma were obviously increased in NE medi-um-dose,high-dose groups,SY high-dose group,compatibility low-dose,medium-dose groups and AFI water decoction medi-um-dose,high-lose groups （P〈0.05）;SP levels in plasma were obviously increased in NA low-dose,medium-dose groups and NE doses groups（P〈0.05 or P〈0.01）;VIP levels were reduced in NA