中文摘要：

目的 研究化疗药物5-Fu对小鼠脾虚证候及肠内容物中短链脂肪酸含量的影响.方法 正常小鼠腹腔注射5-Fu后统计小鼠摄食量、体质量、腹泻等数据,观察其脾虚证候表现,同时通过做HE染色,肠黏膜β-D-半乳糖苷酶活性等评价其胃肠道毒性,并检测肠内容物中短链脂肪酸的含量,探讨5-Fu对肠道菌群的影响.结果 与正常组相比5-Fu引起小鼠摄食减少、体质量下降、腹泻指数增加,病理切片结果表明小肠绒毛变短,病变程度增加,血浆中TNF-α和IL-6含量增加,肠黏膜β-D-半乳糖苷酶活性降低,肠内容物中乙酸、丁酸、戊酸浓度显著降低.结论 5-Fu可致小鼠脾虚证候,对肠黏膜及肠道菌群损伤明显,提示在应用化疗药物5-Fu时,可联合应用健脾中药,减低其胃肠道毒副作用.

英文摘要：

OBJECTIVE To study the effects of 5-Fu on mice with spleen deficiency syndrome and the content of short chain fatty acid in intestine.METHODS After the injection of 5-Fu in abdomen,data such as food intake,body weight changes,diarrhea and other information were collected to observe symptoms due to spleen deficiency.Endo-intestinal toxicity of 5-Fu were evaluated by HE staining and detecting beta-D-galactose glucoside enzyme activity in intestinal mucosa at the same time.The content of short chain fatty acids in intestinal contents were also determined to investigate the influences of 5-Fu on the intestinal flora.RESULTS Compared with control group,the spleen deficiency syndrome may induced by 5-Fu,the mice appeared the symptoms,such as poor appetite,weight loss and diarrhea,and it was shown in the physiological section that the intestine villis significantly shortened,the pathological changes further developed,the contents of TNF-α and IL-6 in plasma increased and the beta-D-galactose glucoside enzyme activity of intestinal mucosa decreased.Contents of acetic acid,butyric acid and pentanoic acid in intestine significantly reduced at the same time.CONCLUSION Fu may induce spleen deficiency syndrome in mice and significantly damage the intestinal mucosa and intestinal flora,which indicate that when 5-Fu is applied,some spleen -strengthening medicine may be used to reduce induced intestinal side effects.