中文摘要：

活动 genomic 岛(GIs ) 能从染色体被切除，然后形成圆形的中介并且被官方补给的内部 integrase 复兴进染色体。一些活动 GIs 能被转变，变化形式，或 transduction 也变成一个新受体房间。integrase 的行动地点通常直接是 flanked GIs 的重复(医生) 。flanking 序列的精确本地化是为决定 GI 的活动性的一个前提。活动 GIs 通常与转移 RNA (tRNAs ) 被联系。基于在 flanking 序列和 tRNA 序列之间的关联，在 Pseudomonas aeruginosa PAO1 的 11 通常认为的活动 GIs 的 flanking 序列， P。aeruginosa PA14， P。fluorescens Pf-5 和 P。fluorescens Pf0-1 被识别。在 11 GIs 之中， Pf0-1GI-1 为安息香酸盐降级负责。PAO1GI-1， Pf5GI-2， Pf5GI-3，和 Pf5GI-4 试验性地被证实从一个染色体被切除形成圆形的中介。integrases 的行动地点是这些 GIs 直接重复。由于不同医生，为 PAO1GI-1， Pf5GI-2， Pf5GI-3 和 Pf5GI-4 的内部 integrase 切地点在 T 环外面被决定 tRNAGly 基因在 tRNASer 基因和 tRNALys 基因的反密码子环外面，并且在 tRNALeu 基因的不对称的 3 结束，分别地。PAO1GI-1 和另外的活动 GIs 可以被变成因为清楚的 flanking 序列，属于不同的数的许多不同紧张。这研究关于 integrases 的行动地点描述基本信息，估计 GIs，和罐头帮助设计和转移的活动性活动 GIs 到候选人紧张。

英文摘要：

Mobile genomic islands （GIs） can be excised from the chromosome, then form a circular intermediate and be reintegrated into the chromosome by the GI internal integrase. Some mobile GIs can also be transferred into a new receptor cell by transformation, conjugation, or transduction. The action sites of the integrase are usually flanked direct repeats （DRs） of the GIs. Accurate localization of the flanking sequences is a precondition for determining the mobility of the GI. Mobile GIs are generally associated with transfer RNAs （tRNAs）. Based on the correlation between flanking sequences and tRNA sequences, the flanking sequences of 11 putative mobile GIs in Pseudomonas aeruginosa PAO1, P. aeruginosa PAl4, P. fluorescens Pf-5 and P. fluorescens Pf0-1 were identified. Among the 11 GIs, Pf0-1GI-1 is responsible for benzoate degradation. PAO1GI-1, Pf5GI-2, Pf5GI-3, and Pf5GI-4 were confirmed experimentally to be excised from a chromosome to form a circular intermediate. The action sites of the integrases are these GIs direct repeats. Due to distinct DRs, cutting sites for the internal integrase of PAO1GI-1, Pf5GI-2, Pf5GI-3 and Pf5GI-4 were determined outside the T-loop of the tRNAGly gene, outside the anticodon loop of the tRNAser gene and tRNALys gene, and at the asymmetric 3＇-end of the tRNALeu gene, respectively. PAO 1GI-1 and other mobile GIs may be transferred into many different strains that belong to different phyla because of the clear flanking sequences. This study describes basic information about the action sites of the integrases, assesses the mobility of GIs, and can help design and transfer mobile GIs to candidate strains.