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Innovation for ascertaining genomic islands in PAO1 and PA 14 of Pseudomonas aeruginosa
  • ISSN号:0023-074X
  • 期刊名称:《科学通报》
  • 时间:0
  • 分类:Q78[生物学—分子生物学] Q939.112[生物学—微生物学]
  • 作者机构:[1]Key Laboratory of Microbial Metabolism, Ministry of Education, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China, [2]College of Life and Environment Science, Shanghai Normal University, Shanghai 200234, China
  • 相关基金:Supported by the National Natural Science Foundation of China (Grant Nos. 30821005 and 30870075), National Key Basic Research and Development Program of China (Grant No. 2009CB118906) and Shanghai Leading Academic Discipline Project (Grant No. B203)
中文摘要:

基于 genomic 岛的三个不同特点,一条新奇途径被开发寻找并且在特殊紧张决定 genomic 岛。在 Pseudomonas aeruginosa PAO1 的二个 genomic 岛和在 Pseudomonas aeruginosa PA14 的 7 个 genomic 岛与这个方法被定义。在 9 个 genomic 岛之中, 4 个岛以前被描绘了,当另外的 5 个岛是开始坚定的时。6 个 genomic 岛的 insert 地点是 tRNA 序列, PA14GI-3 的直接重复相对 tRNA 列伊 , 和 PA14GI-2 的直接重复在 3 ′ 双性人结束功能的 GMP synthase/glutamine amidotransferase。PA14GI-4 的仅仅直接的重复不是清楚的。在 5 个发现得最新的 genomic 岛之中, PA14GI-2 是与 Hg 2+ 举起有关的一个 genomic 岛,这被建议, PA14GI-3 是一个能分泌的活动 genomic 岛, PA14GI-6 是一个致病力岛,并且 PA14GI-1 和 PA14GI-5 的功能不是清楚的。最后,在 PAO1GI-1, PA14GI-5 和 PA14GI-7 的酷氨酸类型 integrases 被分析,并且他们的绑定和限制地点被预言。

英文摘要:

Based on three distinct traits of genomic islands, a novel approach was developed to search for and determine genomic islands in special strains. Two genomic islands in Pseudomonas aeruginosa PAO1 and 7 genomic islands in Pseudomonas aeruginosa PA14 were defined with this method. Among the 9 genomic islands, 4 islands had been characterized before, while the other 5 islands were initially determined. The insert sites of 6 genomic islands are tRNA sequences, direct repeats of PA14GI-3 are relative to tRNA^Leu, and direct repeats of PA14GI-2 are at the 3' end of bifunctional GMP synthase/ glutamine amidotransferase. Only direct repeats of PA14GI-4 are not clear. Among the 5 newly-found genomic islands, it was supposed that PA14GI-2 is a genomic island related to Hg^2+ uptake, PA14GI-3 is a secretory activity genomic island, PA14GI-6 is a pathogenicity island, and functions of PA14GI-1 and PA14GI-5 are not clear. Finally, the tyrosine type integrases in PAOIGI-1, PA14GI-5 and PA14GI-7 were analyzed, and their binding and restriction sites were predicted.

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期刊信息
  • 《科学通报》
  • 北大核心期刊(2011版)
  • 主管单位:中国科学院
  • 主办单位:中国科学院
  • 主编:周光召
  • 地址:北京东黄城根北街16号
  • 邮编:100717
  • 邮箱:csb@scichina.org
  • 电话:010-64036120 64012686
  • 国际标准刊号:ISSN:0023-074X
  • 国内统一刊号:ISSN:11-1784/N
  • 邮发代号:80-213
  • 获奖情况:
  • 首届国家期刊奖,中国期刊方阵“双高”期刊,第三届中国出版政府奖
  • 国内外数据库收录:
  • 美国化学文摘(网络版),美国数学评论(网络版),美国工程索引,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),中国北大核心期刊(2000版)
  • 被引量:81792