基于 genomic 岛的三个不同特点,一条新奇途径被开发寻找并且在特殊紧张决定 genomic 岛。在 Pseudomonas aeruginosa PAO1 的二个 genomic 岛和在 Pseudomonas aeruginosa PA14 的 7 个 genomic 岛与这个方法被定义。在 9 个 genomic 岛之中, 4 个岛以前被描绘了,当另外的 5 个岛是开始坚定的时。6 个 genomic 岛的 insert 地点是 tRNA 序列, PA14GI-3 的直接重复相对 tRNA 列伊 , 和 PA14GI-2 的直接重复在 3 ′ 双性人结束功能的 GMP synthase/glutamine amidotransferase。PA14GI-4 的仅仅直接的重复不是清楚的。在 5 个发现得最新的 genomic 岛之中, PA14GI-2 是与 Hg 2+ 举起有关的一个 genomic 岛,这被建议, PA14GI-3 是一个能分泌的活动 genomic 岛, PA14GI-6 是一个致病力岛,并且 PA14GI-1 和 PA14GI-5 的功能不是清楚的。最后,在 PAO1GI-1, PA14GI-5 和 PA14GI-7 的酷氨酸类型 integrases 被分析,并且他们的绑定和限制地点被预言。
Based on three distinct traits of genomic islands, a novel approach was developed to search for and determine genomic islands in special strains. Two genomic islands in Pseudomonas aeruginosa PAO1 and 7 genomic islands in Pseudomonas aeruginosa PA14 were defined with this method. Among the 9 genomic islands, 4 islands had been characterized before, while the other 5 islands were initially determined. The insert sites of 6 genomic islands are tRNA sequences, direct repeats of PA14GI-3 are relative to tRNA^Leu, and direct repeats of PA14GI-2 are at the 3' end of bifunctional GMP synthase/ glutamine amidotransferase. Only direct repeats of PA14GI-4 are not clear. Among the 5 newly-found genomic islands, it was supposed that PA14GI-2 is a genomic island related to Hg^2+ uptake, PA14GI-3 is a secretory activity genomic island, PA14GI-6 is a pathogenicity island, and functions of PA14GI-1 and PA14GI-5 are not clear. Finally, the tyrosine type integrases in PAOIGI-1, PA14GI-5 and PA14GI-7 were analyzed, and their binding and restriction sites were predicted.