中文摘要：

根据DndB蛋白表达载体的特性,建立了一套集Ni2＋-NTA亲和层析、Source Q阴离子交换层析和Superdex 200分子筛层析于一体的纯化体系,纯化得到高纯度的DndB蛋白,可用于后续的蛋白质结构生物学和酶学特性研究,为阐明DndB蛋白的功能以及DNA硫修饰的机理打下了良好的基础。

英文摘要：

According to the character of DndB expression vector, an appropriate purification system consisting of Ni2＋-NTA affinity chromatography, Source Q anion-exchange chromatography and Superdex 200 gel filtration chromatography was built to purify DndB protein with high purity for structure biology and enzymology study. This work laid a foundation for functional study of DndB protein and mechanistic study of DNA sulfur modification.