中文摘要：

目的初步探讨p53Arg72Pro不同基因型的生物学功能以及在新疆维吾尔族宫颈癌发生机制中的相关作用。方法采用免疫组化的方法检测110例维吾尔族宫颈癌和45例维吾尔族正常宫颈组织中p53基因第四外显子第72位密码子Arg／Pro（p53Arg72Pro）三种基因型Arg／Arg、Pro／Arg、Pro／Pro型中P21^WAF1/CIP1、Bax蛋白的表达。结果在维吾尔族宫颈癌组织中P21^WAF1/CIP1、Bax蛋白的阳性表达率均高于正常宫颈组织，其表达差异有统计学意义（P〈0．05），同时在p53Arg72Pro三种基因型中Pro／Pro型的Bax蛋白阳性高表达率最高66．67％（10／15），其表达差异有统计学意义（P〈0．05）。而P21^WAF1/CIP1蛋白阳性表达率在Arg／Arg型中最高58．5％（24／41），但其差异并无统计学意义（P〉0．05）。结论p53Arg72Pro三种基因型在诱导细胞凋亡、转录激活以及调节细胞周期方面可能存在差异，p53Arg72Pro多态性可能通过不同的机制引起宫颈癌的发生。

英文摘要：

Objective To investigate the biological functions and carcinogenic mechanism of the different genotypes of p53 exon 4 codon 72 of cervical carcinoma in Uigur women. Methods Immunohistochemical staining was used to detect the expression of P21^WAF1/CIP1 and Bax protein of the 110 patients and 45 controls. Results It showed that the expression of P21^WAF1/CIP1 and Bax protein in cervical carcinoma were higher than the normal controls, these differences were significant （P〈0.05）. The ratio of the expression of Bax protein in homozygous proline was 66.67%, which was the highest in three genotypes, and there were significant differences （P〈0.05）. Whereas the expression of P21^WAF1/CIP1 in homozygous arginine was higher than the other genotypes, but there were no significant differences （P〉0.05）. Conclusions We assumed that the functions of promoting cellular apoptosis, transcriptional activation and accommodating cell cycle have distinctions in different genotypes, and the two genotypes may have the different carcinogenic mechanism to develop cervical carcinoma.