中文摘要：

目的探讨HPV16 E6对p53 Arg72Pro不同基因型的p53蛋白及其功能相关蛋白表达的影响，为研究宫颈癌的致癌机制提供理论依据。方法用脂质体法将真核表达载体pcDNA3．1／myc-His（-）A-HPVl6E6转染p53Arg和p53Pro细胞，通过免疫细胞化学方法对转染前后两种细胞内p53、p21、Bax、Ki-67蛋白进行检测。结果转染后两种细胞内p53、Bax、Ki-67蛋白的表达均受到影响，但p21蛋白变化不大。结论两种细胞内p53蛋白均可被降解，被降解后两者抑制细胞增殖能力和诱导细胞凋亡能力降低的程度不同，通过不同机制导致宫颈癌的发生。

英文摘要：

Objective To discuss correlation between HPV16 E6 and p53Arg72Pro polymorphisms, it provided academic evidence for the mechanism of cervical cancer. Methods pcDNA 3.1/myc-His （-） A HPV16 E6 was transfected into p53 codon 72 genotype cell lines, Arg homozygous （SK BR-3） and Pro homozygous （MIA PaCa-2）. Immunoeytochemistry was used to detected the expression of protein of p53,p21,Bax,Ki67 proteins in these cells which were transfected by HPV16 E6. Results After being transfected, the expression of p53,Bax,Ki-67 were effected in those cells, expect for p21 protein. Conclusion After the eukaryotic expression vector pcDNA 3.1/myc-His （-） A-HPV16 E6 was transfected into the Arg homozygous and Pro homozygous cells, the p53Arg and p53Pro proteins were all degraded by HPV16 E6. And the ability of proliferation and apoptosis of the cells were depressed to a different degree.