中文摘要：

目的p73G4A多态性与新疆维族HPV相关宫颈癌发生的相关性；探讨PCR-CTPP在SNP检测及分析中的应用。方法应用PCR、PCR-RFLP以及PCR-CTPP技术对101例维族宫颈癌和100例维族正常宫颈组织的p73G4A多态性分布及HPV16的感染情况进行检测。结果p73G4A多态性3种基因型GC／GC、AT／AT和GC／AT在维吾尔族宫颈癌中所占比例差异无显著性（χ^2=2．268，P〉0.05）；HPV16DNA检测显示，HPV16感染在宫颈癌及对照组中分布经统计学分析差异有显著性（χ^2=116．837，P〈0.05）；p73G4A多态性3种基因型在HPV16阳性组和阴性组中分布，经统计学分析差异无显著性（χ^2=1．820，P〉0．05）；采用PCR-CTPP技术检测分析维族宫颈癌p73G4A多态性，与PCR-RFLP技术检测结果一致。结论p73G4A多态性可能与维族宫颈癌无相关性；PCR-CTPP技术经济、省时、操作简单，结果稳定可靠，对于SNP以及基因点突变研究具有较高的应用价值。

英文摘要：

[Objective] To investigate the association between p73G4A polymorphism and HPV-associated cervical carcinoma in Uigur women in Xinjiang. To study the application of PCR-CTPP in SNP analysis. [Methods] The distribution of HPV16 and the p73 exon2 polymorphism of 101 cases of cervical carcinoma and 100 cases of normal controls were detected by using PCR, PCR-RFLP and PCR-CTPP. [Results] Difference of genotype frequencies of GC/GC, AT/AT and GC/AT in p73 polymorphism between the cervical carcinomas and the controls was not significant statistically （χ^2=2.268, P 〉0.05）. Difference of HPV16 positive rate between the cervical carcinomas and the controls was significant statistically （χ^2=116.837, P 〈0.05）. Difference of genotype frequencies of p73 polymorphism between HPV16 positive group and HPV16 negative group was not significant statistically（χ^2=1.820, P 〉 0.05）, PCR-CTPP is used to detection of polymorphism of p73 exon 2 G4A in cervical carcinoma of Uignr women, which was compared with the PCR-RFLP. [Conclusion] p73 G4A polymorphism may not be associated with cervical carcinoma in Uigur women in Xinjiang, PCR-CTPP is an inexpensive, easy time-saving and reliable method compared with other methods.