中文摘要：

本试验分别采用高脂饲料和高脂饲料复合四氯化碳对C57BL/6J小鼠进行诱导,研究建立非酒精性脂肪肝模型的条件。本试验将C57BL/6J小鼠分成高脂组、高脂复合四氯化碳组（复合组）和正常组。各组分别于饲喂4、6、8周处死5只,称小鼠体重与肝重,采血测血清中生化指标情况,并取肝脏做H.E.染色、油红O染色。通过肝指数、生化与病理检查等指标,评价非酒精性脂肪肝的进程。结果显示,随着饲喂时间加长,高脂组动物肝脏脂肪变性加重;复合组动物除肝脏脂肪变性外,出现纤维化和明显的炎性浸润;4周时,高脂组中脂肪变性比复合组明显。6～8周时高脂组和复合组中生化指标明显高于正常组。本试验经饲喂高脂饲料成功复制了小鼠非酒精性脂肪肝模型。

英文摘要：

To establish a non-alcoholic fatty liver animal model which is respectively induced by high-fat diet and high-fat diet with CC14. C57BL/6J mice were randomly divided into the control group, the high-fat diet model group and the high-fat diet with CC14 model group（compound groups）. For each group, body weight, liver weight and the serum ATL, AST, GGT, TG, Chol were measured and assayed at 4th,6th,Sth week. And some liver tis- sue taken from killed mouse were needed for histopathological examination. In high-fat diet group, the liver steatosis was step by step. Besides the steatosis, there were obvious inflammatory infiltration and fibrosis in compound group. At 4th week, the steatosis was more obvious in high-fat diet group than that of compound group. From 6th to 8th weeks,the serum biochemical index in high-fat diet and compound groups were higher than that in control group. We have successfully established the model of non-alcoholic fatty liver in C57BL/6J mouse by high-fat diet.