中文摘要：

目的探讨过氧化物酶增殖活化受体α（PPARα）和解偶联蛋白2（UCP-2）在非酒精性脂肪肝病（NAFLD）小鼠肝组织中的表达。方法雄性成年ICR小鼠30只随机分成3组，每组10只，即对照组（普通饲料）、模型A组（普通饲料4周＋高脂饲料4周）、模型B组（高脂饲料8周），分别测定肝脏指数并制作肝脏病理切片，采用反转录．聚合酶链反应（RT—PCR）方法，测定小鼠肝组织中PPARet和UCP-2mRNA表达。结果对照组、模型A、B组小鼠肝指数分别为（3．89±0．87）％、（7．42±0．95）％、（9．38±1．07）％，模型组小鼠肝指数均高于对照组（P〈0．01），模型小鼠肝脏脂肪变性明显；模型A、B组小鼠肝组织中PPARetmRNA表达量分别为（0．63±0．33）、（0．45±0．19），低于对照组的（1．16±0．27）（P〈0．01），模型A、B组小鼠肝组织中UCP-2mRNA表达量分别为（0．67±0．76）、（0．89±0．52），高于对照组的（0．25±0．13）（P〈0．01）。结论发生NAFLD的小鼠肝组织中PPARd和UCP-2mRNA表达异常。

英文摘要：

Objective To explore the expressions of peroxisome proliferator-activated receptor α （PPARα） and un- coupling proteins 2 （ UCP-2 ） in hepatic tissue in the mice with non-alcohlic fatty liver disease （ NAFLD ）. Methods Thirty mature male ICR mice were randomly divided into three groups （ 10 in each group）. The mice in control group were fed with normal diet; the mice in experimental group A were fed with normal diet for 4 weeks; and then with high- fat diet for 4 weeks; and the mice in experimental group B were fed with high-fat diet for 8 weeks. Liver index were measured. The expressions of PPARa and UCP-2 mRNA in hepatic tissue were examined with reverse transcription poly- merase chain reaction（ RT-PCR）. Results The liver index for the mice in control group, experimental group A and ex- perimental group B were 3. 89 ±0. 87,7. 42 ±0. 95 ,and 9. 38 ±1.07 ,respectively. In the mice of experimental group,the liver index was significantly higher than that of the control mice（ P 〈 0.01 ）. The steatosis of hepatic tissue in experimen- tal group was obvious. PPARa mRNA expressions in hepatic tissue of mice of experimental group A（0. 63 ±0. 33 ）and experimental group B （ 0.45 ±0. 19 ） were dramatically decreased compared to normal group （ 1.16 ± 0. 27 ） （ P 〈 0. 01 ）. UCP-2 mRNA expressions in hepatic tissue of mice of experimental group A （ 0. 67 ± 0. 76 ） and experimental group B （ 0. 89 ± 0. 52 ） were dramatically increased compared to normal group （ 0. 25 ± 0. 13 ） （ P 〈 0. 01 ）. Conclusion The ex- pression of PPAR a and UCP-2 in hepatic tissue of NAFLD mice are abnormal.