目的探索湖北钉螺体内单位体重软体组织葡萄糖含量的季节性变化。方法于2012年2月-2013年1月每月初,采集安徽省芜湖市青弋江滩的湖北钉螺若干,并置于-80℃冰箱冷冻保存。采用常规方法压碎螺壳,鉴别雌雄,分别收集雌雄螺软体组织,己糖激酶法测定葡萄糖含量,并对结果进行统计学分析。结果 2-7月,钉螺体内葡萄糖含量总体呈下降趋势。雌螺和雄螺的葡萄糖含量分别在3月和7月降至全年最低值,分别为1.87μg/mg和3.70μg/mg,并均于8月出现回升,9月时葡萄糖含量达到全年最高值,分别为57.38μg/mg和44.39μg/mg,之后又出现持续下降,至翌年1-2月葡萄糖含量再次出现回升。结论湖北钉螺体内葡萄糖含量随着季节变化而出现有规律的变化。
Objective To study the seasonal changes of glucose levels per unit soft tissue of Oncomelania hupensis. Methods O. hupensis snails were collected from the beach of the Qingyi River in Wuhu City,Anhui Province from August 2012 to July 2013. They were kept in minus 80 ℃ refrigerator immediately. The male snails were distinguished from female,and their soft tissues were collected separately after crushing their shells. The hexokinase method was used to measure the glucose concentrations,and the results were analyzed statistically. Results The contents of glucose decreased from March to July. The lowest glucose content in the female was 1.87 μg/mg in March,and that in male was 3.70 μg/mg in July. Both of them increased from August and reached peak levels in September(♀ = 57.38 μg/mg,♂ = 44.39 μg/mg),and then gradually decreased from October to next January and increased in February. Conclusion The contents of glucose have seasonal changes regularly in O. hupensis.