中文摘要：

目的采用染色法判别日本血吸虫尾蚴的生命状态。方法以0.4%台盼蓝、0.5%M.E.B、0.5%伊红、0.5%甲基蓝、0.05%中性红等5种染色液分别对死活日本血吸虫尾蚴染色5 min,显微镜下观察着色情况。结果台盼蓝、M.E.B、伊红、中性红4种染色液可使死尾蚴着色,而甲基蓝染液对其不着色;5种染液均不能使活尾蚴着色。结论0.4%台盼蓝、0.5%M.E.B、0.5%伊红、0.05%中性红染液染色可判别日本血吸虫尾蚴的生命状态。

英文摘要：

Objective To determine the viability of Schistosoma japonicum cercariae by staining. Methods Schistosoma japonicum cereariae were stained by 0.4% trypan blue, 0.5% methylene blue-eosin-borax （M.E.B）, 0.5% eosin, 0.5% methy- lene blue and 0.05% neutral red, respectively, for 5 min, then they were observed under a stereoscopic microscope. Results The dead eercariae were stained in the trypan blue, M.E.B, eosin and neutral red, but unstained in the methylene blue. The vi- tal cercariae were unstained in all the five kinds of dyes. Conclusion The staining methods by using 0.4% trypan blue, 0.5%M.E.B, 0.5% eosin and 0.05% neutral red can be used to determine the viability of S. japonicum cercariae.