中文摘要：

目的：研究RNA干扰抑制宣威女性肺癌细胞XWLC-05中转录因子sox4的表达对细胞生长、侵袭和迁移能力的影响。方法：从不同类型肺癌细胞株中,通过实时荧光定量PCR法筛选出高表达sox4的宣威女性肺癌细胞XWLC-05作为研究细胞。将sox4-siRNA转染入XWLC-05细胞后,采用实时荧光定量PCR和蛋白质印迹法检测sox4表达水平的变化。分别采用MTS法、细胞划痕实验和Transwell细胞侵袭实验分析抑制sox4表达对XWLC-05细胞体外增殖、迁移和侵袭能力的影响。FCM评估抑制sox4表达对细胞凋亡和细胞周期的影响。结果：转染sox4-siRNA的实验组细胞在转染后24h时与空白对照组和阴性对照组相比,sox4mRNA及蛋白表达水平差异均无统计学意义（P〉0.05）;转染后48h和72h时与空白对照组和阴性对照组相比,sox4mRNA及蛋白表达水平明显降低（P〈0.05）。转染sox4-siRNA的XWLC-05细胞的增殖活性与空白对照组和阴性对照组相比,差异均无统计学意义（P值分别为0.059和0.113）,而其迁移能力和侵袭能力却明显低于空白对照组（P值均为0.000）和阴性对照组组（P值分别为0.023和0.000）。转染sox4-siRNA的XWLC-05细胞的凋亡率明显高于空白对照组和阴性对照组（P值均为0.000）,但sox4-siRNA转染组的细胞增殖指数与空白对照组和阴性对照组相比,差异均无统计学意义（P值分别为0.168和0.225）。结论：转录因子sox4可能通过抑制凋亡并促进肿瘤细胞的迁移和侵袭能力,在宣威女性肺癌的生长和转移中发挥作用。

英文摘要：

Objective： To evaluate the effects of RNA interference targeting transcription factor sox4 on the growth and the abilities of invasion and metastasis of Xuanwei female lung cancer XWLC-05 cells after transfection with sox4-small-interfering RNA （siRNA）. Methods： The expression of sox4 among different types of lung cancer cell lines was detected by real-time fluorescent quantitative PCR, and eventually the Xuanwei female lung cancer XWLC-05 cell line was selected as the object of the sequential study. Then the XWLC-05 cells were transfected with sox4-siRNA, and the change of sox4 gene expression level was detected by real-time fluorescent quantitative PCR and Western blotting. In vitro proliferation and the abilities of migration and invasion of XWLC-05 cells transfected with sox4-siRNA were determined by MTS assay, cell wound healing assay and Transwell invasion assay, respectively. Flow cytometry was used to assess the apoptosis and the distribution of cell cycle after the inhibition of sox4 expression. Results： Compared with the blank and negative controls, the XWLC-05 cells transfected with sox4-siRNA showed no significant differences in the expressions of sox4 mRNA and protein at 24 h after transfection （P 〉 0.05）. However, the expressions of sox4 mRNA and protein in XWLC-05 cells were significantly down-regulated at 48 h and 72 h after transfection with sox4-siRNA （P 〈 0.05）. The Proliferative activity of XWLC-05 cells transfected with sox4-siRNA was not significant different from those of the blank control cells （P = 0.059） and the negative control cells （P = 0.113）. The capacities of migration and invasion of sox4-siRNA-transfected XWLC-05 cells were significant decreased in vitro, as compared with those of the blank （P = 0.000; P = 0.000） and the negative control （P = 0.023; P = 0.000） cells. The apoptosis rate of sox4-siRNA-transfected XWLC-05 cells was significantly higher than those of the blank and the negative control cells （P = 0.000; P = 0.000）. However, th