中文摘要：

目的体外实验研究β4整合素shRNA对非小细胞肺癌H460SM细胞增殖的抑制作用,为非小细胞肺癌早期诊断和治疗提供新的靶点。方法实时定量RT-PCR验证β4整合素表达水平的变化,显微镜下观察细胞形态的变化。通过细胞计数和MTS实验分析抑制β4整合素表达对H460SM细胞增殖的影响;流式细胞术分析抑制β4整合素表达对H460SM细胞凋亡和细胞周期的影响。结果与阴性对照组（H460SM-NS）比较,稳定表达β4整合素shRNA的实验组（H460SM-68、H460SM-71）细胞β4整合素表达水平明显降低（P〈0.01）;与阴性对照组比较,实验组细胞的增殖受到明显抑制（P〈0.05）;实验组细胞凋亡率明显高于阴性对照组,差异有统计学意义（P〈0.01）;实验组细胞增殖指数PI明显低于阴性对照组（P〈0.05）,G0/G1期细胞数明显高于阴性对照组（P〈0.05）,提示抑制β4整合素的表达,细胞周期可能被阻滞在G1/S检测点,导致细胞增殖受到抑制。结论抑制β4整合素的表达,可以抑制非小细胞肺癌H460SM细胞增殖,并可能诱导H460SM细胞凋亡。癌细胞增殖受到抑制可能与细胞周期调控有关。抑制β4整合素的表达,可能不影响非小细胞肺癌细胞凋亡。

英文摘要：

Objective To investigate the effect ofβ4integrin gene silencing on the proliferation of human lung carcinoma variant cell line H460SM was investigated in vitro to further provide new targets for early diagnosis and treatment of non-small cell lung cancer.Methods β4integrin gene expression was detected by quantitative reverse transcriptase PCR,and the cellular morphology was identified by microscope.Tumor cells growth was detected by MTS assay in vitro.Furthermore,the changes of cell cycle and apoptosis were analyzed by flow cytometry.Results Compared with negative control of shRNA-transduced H460SM cells（H460SM-NS）,β4integrin gene expressions in two different H460SM cells with integrin β4-specific shRNA（H460SM68and H460SM71）were statistically down-regulated（P 〈0.01）.The growth rates of H460SM cells with stably expressing integrinβ4-specific shRNA significantly were decreased compared with negative control（P〈0.05）.Knockdown ofβ4integrin by two different shRNAs led to significantly increase in apoptotic rate as compared with negative control（P〈0.01）.The proportion of H460SM cells with integrinβ4-specific shRNA in the G0/G1phase was significantly higher than that of negative control,suggesting that the cell proliferation was inhibited as blocking at G1/S phase（P〈0.05）.PI value of H460SM cells with integrinβ4-specific shRNA was also lower than that of negative control（P 〈0.05）.Conclusion Knockdown ofβ4integrin expression in non-small cell lung cancer cells could inhibit in vitro cell proliferation.Furthermore,β4integrin shRNA could induced cell apoptosis in H460SM cells.The possible mechanism of H460SM cell growth inhibition resulted fromβ4integrin might be related to cell cycle arrest.