中文摘要：

目的：探讨急性心肌梗死（AMI）后外周血循环单个核细胞（MNC）中1型多聚二磷酸腺苷核糖合成酶（PARP-1）的活性与肿瘤坏死因子-α（TNF-α）、白细胞介素-10（IL-10）水平的关系。方法：选取AMI组患者46例，稳定型心绞痛组10例，正常对照组10例。用三氯乙酸沉淀法测定MNC中PARP-1的活性；酶联免疫吸附测定法测定血浆中IL-10和TNF-α的水平。结果：所有AMI组患者MNC中PARP-1的活性均显著升高，为正常对照组的4．08倍、稳定型心绞痛组的4.47倍。所有AMI患者血浆中TNF-α、IL-10的水平均显著升高。AMI组患者外周血循环MNC中PARP-1的活性与血浆TNF-α、IL-10的水平呈正相关。结论：患者AMI后外周血血浆中TNF-α、IL-10水平的增高与MNC中PARP-1的活化显著相关。PARP-1可能是免疫炎症系统激活过程中的重要调节因子。

英文摘要：

Objective： To explore the relationship between the poly （ADP-ribose） polymerase-1 （PARP-1） activation of circulated mononuclear cell （MNC） and the concentrations of tumor necrosis factor-α （TNF-α） or interleukin-10 （IL-10） in plasma in patients with acute myocardial infarction （AMI）. Method：There were 46 patients with AMI, 10 with stable angina and 20 as normal control enrolled in this study. PARP activity was measured with trichloroacetonitrile （TCA） precipitation method. Plasma IL-10 and TNF-α were measured with ELISA. Result：PARP-1 activity of circulating MNC increased significantly in all post-AMI patients. It was about 4.08 times higher than the healthy cases or 4.47 times higher than the stable angina pectoris cases. Plasma TNF-α, IL-10 increased significantly in all post AMI patients. PARP-1 activity of MNC correlated positively with plasma TNF-α level, IL-10 level respectively. Conclusion： Increases in plasma TNF-α and IL-10 are associated with the activation of PARP-1 of circulating MNC in patients with AMI, suggesting that PARP-1 might be an important regulatory factor in the inflammatory response.