中文摘要：

目的研究用化学发光成像的方法观察脐带间充质干细胞转染CMV-Luciferase—PGK—Puro慢病毒后的情况，代替活体成像仪的可行性。方法用CMV-Luciferase-PGK—Puro慢病毒转染树嗣脐带间充质干细胞，用最适浓度的嘌呤霉素筛选，存活的细胞用6孔板的3个孔培养，贴壁后，3个孔依次加入底物D-荧光素钾盐，用化学发光成像仪拍照，再用软件进行活体成像转换。将转染成功的细胞注入麻醉后的树嗣皮下，树朐静脉注射底物。结果细胞加入底物后有生物发光，发光强度随底物作用时间延长而减弱。树驹皮下也观察到发光细胞。结论CMV—Luciferase-PGK—Puro慢病毒能成功转染树鼢脐带间充质干细胞，转染成功的细胞用于动物模型治疗后，可进行活体成像，观察细胞在动物体内的分布。

英文摘要：

objective To study the feasibility of chemiluminescence imaging instead of living imaging for observing the conditions after tree shrews umbilical cord mesenchymal stem cells （UC-MSCs） are transfected with CMV-Luciferase-PGK-Puro lentivirus. methods The tree shrew UC-MSCs transfected with CMV-Luciferase-PGK-Puro lentivirus were screened with puromycin of optimum concentration. The survival cells were cultured with three holes of six-hole plate. Upon adherence, the substrate d- luciferin potassium salt was successively added to the three holes. A picture was taken by chemiluminescence imager, after which living imaging conversion was conducted. The cells successfully transfected were injected under the skin of postanesthetic tree shrew, which were injected with substrate by intravenous injection, Results Upon the addition of substrate, the cells had bioluminescence; the luminousintensity weakened with the extension of substrate action time. Subcutaneous luminous cells were also observed under the skin of tree shrew. Collusion CMV-Lueiferase-PGK-Puro lentivirus may successfully transfeet tree shrews UC-MSCs. Successfully transfeeted cells used for the treatment of animal models can undergo living imaging to observe the distribution of cells in the animal body.