中文摘要：

该研究发现，鸡卵细胞提取液有促进239T细胞多能因子表达增加的作用，将在细胞生物学有广泛的应用价值。将鸡卵清提取液分离为大于10kDa和小于10kDa的组分，用各提取液50％的终浓度培养293T细胞5d，用流式细胞术检测多能因子的表达。结果表明，鸡卵清提取液及不同组分都有促进293T细胞多能因子表达增加的作用，而大于10kDa的组分作用最明显，差别有统计学意义（P〈0．01）。结果提示，鸡卵清提取液有促进239T细胞多能因子表达增加的作用，鸡卵清提取液中可能存在促进体细胞重编程作用的物质。

英文摘要：

This work was aim to investigate the effect of the chicken egg-white extract on the expression of pluripotent factors of 239T cells. We isolated chicken egg-white extract into 2 fractions, greater or less than 10 kDa fractions. And 2 different fractions were used with a final concentration of 50% to culture 293T cells for 5 days, respectively. And then, the levels of pluripotent factors in the 293T cells were detected by flow cytometry. The results showed that the chicken egg-white extract and its 2 fractions had the roles of increasing pluripotent factors in 293T cells. And the fractions of greater than 10 kDa had the most obvious effects with statistically significant （P〈0.01）. In conclusion, chicken egg-white extract and its fractions increased the expression of pluripotent factors in somatic cells. The chicken egg-white extract might have some substances to promote to reprogramme somatic cells.