中文摘要：

背景：多器官功能障碍综合征为当今临床治疗的一大难题,骨髓间充质干细胞移植为其提供了一个新的治疗研究方向。目的：观察骨髓间充质干细胞移植治疗多器官功能障碍综合征的疗效。方法：按1 mg/kg剂量股静脉注射内毒素建立大鼠多器官功能障碍综合征模型,将存活的大鼠随机分为治疗组和模型组,治疗组大鼠尾静脉注射500μL（5×106）第3代骨髓间充质干细胞,对照组尾静脉注射等体积的生理盐水;治疗5 d后行血常规、血生化、凝血功能检测,取血后处死大鼠取肝脏、肾脏、心脏、脾脏进行苏木精-伊红染色病理学观察。结果与结论：（1）移植治疗1周后,与模型组相比,治疗组肝功能、凝血功能各项指标明显得到改善（P〈0.05）,白细胞计数更接近正常水平（P〈0.05）;（2）模型组心、肝、脾、肾脏均出现不同程度的炎症性病变和坏死,治疗组炎性浸润和坏死性病变明显减轻;（3）结果提示,骨髓间充质干细胞对多器官功能障碍综合征模型大鼠具有明显的治疗作用。

英文摘要：

BACKGROUND： Clinical treatment of multiple organ dysfunction syndrome has been a big problem today, while the treatment of bone marrow mesenchymal stem cells may provide a new research direction. OBJECTIVE： To investigate the therapeutic effects of bone marrow mesenchymal stem cells transplanted into multiple organ dysfunction syndrome models.METHODS：According to body weight of rats,endotoxin in the 1 mg/kg dose was injected into the femoral vein to build the multiple organ dysfunction syndrome models in rats,and the surviving rats were randomly divided into treatment and control groups.Then the rats in the treatment group were injected intravenously third-generation bone marrow mesenchymal stem cells（5×106）,while rats in the control group injected intravenously with an equal volume of saline.After 5 days of treatment,the blood routine examination,blood biochemistry and coagulation tests were conducted,and then the liver,kidney,heart,and spleen of rats were captured to observed pathological changes through the hematoxylin-eosin staining.RESULTS AND CONCLUSION：One week after cell transplantation,liver function and biochemical indicators were improved significantly in the treatment group compared with the control group（P〈0.05）,and the white blood cell count in the treatment group was closer to normal levels（P〈0.05）.Hematoxylin-eosin staining results showed that in the multiple organ dysfunction syndrome rats appeared to have different degrees of inflammatory lesions and necrosis in the heart,liver,spleen,and kidneys,while inflammatory infiltration and necrotic lesions decreased significantly in the treatment group compared with the control group.To conclude,bone marrow mesenchymal stem cells exert remarkable therapeutic effects on multiple organ dysfunction syndrome.