中文摘要：

利用Trichoderma sp.W2所产的嗜温耐乙醇β-葡萄糖苷酶及耐高温酵母Kluyveromyces marxianus NCYC 587,以气爆秸秆为原料进行高温同步糖化发酵。研究结果表明：在42℃条件下,接种体积分数10%,底物质量分数15%,发酵pH值为4.8,β-葡萄糖苷酶添加量为30 U/g底物条件下发酵效果最好。NCYC 587能迅速利用预水解产生的葡萄糖发酵并积累乙醇,同时能利用部分木糖,但在发酵后期,葡萄糖利用完全后会代谢利用一定量的乙醇,致使发酵过程中乙醇质量浓度始终维持在一个相对较低的水平。乙醇最高质量浓度达到20.56 g/L,乙醇产率达80.64%。添加嗜温耐乙醇β-葡萄糖苷酶于高温同步糖化发酵能有效解决纤维素酶解发酵过程终端产物抑制的难题。

英文摘要：

To develop a high efficiency simultaneous saccharification and fermentation（SSF） method,steam-exploded corn stalks as raw material,ethanol-resistant thermostable β-glucosidase from Trichoderma sp.W2 and thermostable yeast Kluyveromyces marxianus NCYC 587 were used in the study on the bioethanol production application.The results show that the optimum fermentation conditions are as follows： inoculum volume fraction 10%,substrate mass fraction 15%,pH=4.8,42 ℃,and the β-glucosidase addition of 30 U for 1 g substrate.Strain NCYC 587 can quickly take advantage of the glucose in pre-hydrolysis and fermentation to produce and accumulate ethanol,furthermore,it′s able to use part of the xylose.In the late fermentation,after the glucose is completely consumed,a certain amount of alcohol is used,which results in the fermentation process of ethanol at a relatively low level.Under the optimum conditions,the thermostable yeast can grow and ferment well,and the highest ethanol mass concentration and ethanol theoretical conversion rate can attain to 20.56 g/L and 80.64% respectively after 24 h of fermentation.Adding ethanol-resistant thermostable β-glucosidase at high temperature SSF can effectively solve the problem of terminal hydrolysate inhibition.