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中文摘要：
酮酸脱羧酶作为异戊醇生物合成的关键酶,不存在于大肠杆菌中。以乳酸乳球菌的基因组DNA为模板,经过PCR扩增得到酮酸脱羧酶基因kivD（rbs）,插入到大肠杆菌高效表达载体pET-28a（＋）上形成pET-kivD（rbs）,重组质粒热击转化进大肠杆菌 BL21（DE3） 中,其成功表达了酮酸脱羧酶。对发酵产物进行分析,检测到了微量的目标产物—异戊醇。
英文摘要：
Keto acid decarboxylase is the key enzyme for biological synthesis of isoamyl alcohol, rarely existed in E. coli. Keto acid decarboxylase gene kivD（rbs） obtained from Lactococcuslactis subsp.Lactis genome DNA through reaction of PCR, was inserted into E. coli high expression vector pET-28a（＋）. The formed pET-kivD（rbs）, was then transformed into E. coli BL21（DE3） by hot blow. The activity of keto acid decarboxylase and isoamyl alcohol were detected in the fermentation broth.
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