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中文摘要：
以大肠埃希菌 MG1655的基因组为模板，通过PCR扩增获得木糖异构酶基因xylA。利用敲除编码对基因转录起负调控作用的lacIq基因的大肠埃希菌/谷氨酸棒杆菌穿梭质粒pEC-XK99E，酶连后转化大肠埃希菌BL21和谷氨酸棒杆菌ATCC 13032。成功构建出了具有大肠埃希菌BL21表达活性的木糖异构酶表达载体Ptrc-xylA。
英文摘要：
The gene of xylose isomerase xylA was obtained taking the genome of E. coli MG1655 as a template and amplified through PCR. Using a shuttle vector pEC-XK99E of E. coli/C. glutamicum after using knock-out code that acted as negative regulation gene lacIq, after enzymic linkage transferred E. coli BL21 and C. glutamicum ATCC13032. Thus successfully constructed an expression vector Ptrc-xylA of xylose isomerase possessing E. coliBl21 expression activity.
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